"
Team:Paris Bettencourt/SID
From 2012.igem.org
Zmarinkovic (Talk | contribs) (→Design) |
Zmarinkovic (Talk | contribs) (→Overview) |
||
| Line 6: | Line 6: | ||
==Overview== | ==Overview== | ||
Provide an overview of this sub-project in a couple of phrases | Provide an overview of this sub-project in a couple of phrases | ||
| + | |||
| + | |||
| + | Bacteria developed mechanisms to kill other bacteria, thus reducing competition between them | ||
| + | |||
| + | in the environment. Some strains of E. coli produce lethal proteins called colicins which | ||
| + | |||
| + | kill other bacteria, including E. coli. Colicins are built out of three main domains which | ||
| + | |||
| + | are also a point of difference among many types of colicins. First domain is responsable for | ||
| + | |||
| + | binding to a receptor on a bacterial membrane, second one is responsable for translocating | ||
| + | |||
| + | the protein from outside to inside of a bacteria and the third one is responsable for | ||
| + | |||
| + | killing bacteria. We are interested in two types of colicins, colicin E2 and colicin D. Both | ||
| + | |||
| + | of them use different binding, translocation and killing mechanisms. Colicin E2 is a DNAse | ||
| + | |||
| + | and colicin D is a RNAse. | ||
| + | |||
| + | We hypothesize that it is possible to use binding and translocation part of colicin as a | ||
| + | |||
| + | "synthetic import domain" onto which we can phuse other proteins in order to import them | ||
| + | |||
| + | into bacteria. Main aim of this part of the project is to phuse the RNAse domain from colicin D to the "synthetic import domain" of colicin E2. By doing this we will obtain a toxin that targets the same receptor and translocation mechanism but kills bacteria on the level of protein synthesis by cleaving tRNA. [link to suicide group]. | ||
| + | |||
| + | In addition to this, we phused restriction enzymes FseI and I-SceI both to the colicin E2 and colicin D "synthetic import domain". These two enzymes are used in different parts of our project [link to restriction group]. We also phused a couple of other proteins to easly test the plausibility of our "synthetic import domain" system. | ||
==Objectives== | ==Objectives== | ||
Revision as of 15:57, 24 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety AssessmentContents |
Overview
Provide an overview of this sub-project in a couple of phrases
Bacteria developed mechanisms to kill other bacteria, thus reducing competition between them
in the environment. Some strains of E. coli produce lethal proteins called colicins which
kill other bacteria, including E. coli. Colicins are built out of three main domains which
are also a point of difference among many types of colicins. First domain is responsable for
binding to a receptor on a bacterial membrane, second one is responsable for translocating
the protein from outside to inside of a bacteria and the third one is responsable for
killing bacteria. We are interested in two types of colicins, colicin E2 and colicin D. Both
of them use different binding, translocation and killing mechanisms. Colicin E2 is a DNAse
and colicin D is a RNAse.
We hypothesize that it is possible to use binding and translocation part of colicin as a
"synthetic import domain" onto which we can phuse other proteins in order to import them
into bacteria. Main aim of this part of the project is to phuse the RNAse domain from colicin D to the "synthetic import domain" of colicin E2. By doing this we will obtain a toxin that targets the same receptor and translocation mechanism but kills bacteria on the level of protein synthesis by cleaving tRNA. [link to suicide group].
In addition to this, we phused restriction enzymes FseI and I-SceI both to the colicin E2 and colicin D "synthetic import domain". These two enzymes are used in different parts of our project [link to restriction group]. We also phused a couple of other proteins to easly test the plausibility of our "synthetic import domain" system.
Objectives
Write here the objectives of your project
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
Present your results
Testing of the system
Experimental setup
Describe the experiment
Results
Present your results
