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Team:Goettingen/week13-3
From 2012.igem.org
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<h2><b>V07_24 </b></h2><br> | <h2><b>V07_24 </b></h2><br> | ||
| - | <b>Analysis of plates and liquid culture V07_20</b><br> | + | <b>2<sup>nd</sup> round: Analysis of plates and liquid culture V07_20</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br>Both the liquid culture and the plates exhibited bacterial growth. 14 clones were transferred to a 5 mL liquid culture (LB + CM) and incubated over night at 37 °C, 180 rpm. The liquid culture was centrifuged at 4800 rpm, 4 °C, 10 min. The pellet was stored at -20 °C.</li> | <li>Experiment: <br>Both the liquid culture and the plates exhibited bacterial growth. 14 clones were transferred to a 5 mL liquid culture (LB + CM) and incubated over night at 37 °C, 180 rpm. The liquid culture was centrifuged at 4800 rpm, 4 °C, 10 min. The pellet was stored at -20 °C.</li> | ||
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</table> | </table> | ||
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| + | <h2><b>V07_25 </b></h2><br> | ||
| + | <b>2<sup>nd</sup> round: Plasmid preparation clones 1-14 for subsequent sequencing</b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br>Plasmid preparation was performd with peqGOLD Plasmid Miniprep Kit (PeqLab) following the user manual. DNA concentrations were determined via nanodrop for subsequent sequencing.</li> | ||
| + | </ul> | ||
| + | <br></td></tr> | ||
| + | </table> | ||
<br> | <br> | ||
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | ||
Revision as of 14:59, 24 September 2012
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#3 Chemoreceptor Library - 13th WeekBack to overview
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