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Team:Bordeaux/Ninth
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Revision as of 10:57, 24 September 2012
Day 39 : 27-08-2012
Today we put the transformants (5 coloines per plate ) on LB precuture.
Day 40 : 28-08-2012
Today we did a miniprep of the precultures. And we runned a gel to test the ligations. IIIAIIB , 5AIVB and IIIDIIE are OK but ICIE will have to be redone.
Day 41 : 29-08-2012
Today we did ligations for IVA5AIVB , 5AIVDIVEIVC , IIAIIBIIC(1)5B ,IAIB , 5AIIIC , ICIE. We inactivated the ligase after 1 hour 20°C incubation and put the samples on the fridge overnight.
Day 42 : 30-08-2012
Today we purified and transformed the ligations by electoporation.
Day 43 : 31-08-2012
Today we put the transformants into a LB preculture and put them at room temperature over the weekend.
