Team:Paris Bettencourt/Delay

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Overview

This part of the design will trigger the DNA degradation mechanism. To do so, we will use two different approaches. The first one, that we called "simple delay system", will be a way to trigger a restriction enzyme, which will cut the plasmid carrying the antitoxin, and thus let the cell sensitive to the toxin produced on the chromosome. In the "sRNA delay system, we use a different strategy. Here, the toxin would be cloned without the Immunity protein, in order to avoid any possible resistance to the toxin due to a non-degraded plasmid. To ensure that the toxin will not be produced while the cells are performing their function, we will use a stationary phase promoter and sRNA repression induced by arabinose.

Simple delay system

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sRNA delay system

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Experiments and results

Characterisation of X

Experimental setup

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Results

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Testing of the system

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Results

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