Team:NTU-Taida/Result

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Revision as of 07:31, 23 September 2012

Project

Testing Hero

Results

Secretion system

In order to evaluate the secretion system, we combined our products and signal peptides (SP1,SP2 and SP3). We de novo synthesized the following peptide genes: signal peptide 1(SP1)-CPP-Flag; signal peptide 2(SP2)-CPP-Flag; signal peptide 3(SP3)-CPP-Flag; SP1-GLP-1. By linking these peptide genes with constitutively-expressed promoter and ribosome-binding site, we use either Escherichia coli K12 DH5α or Escherichia coli BL-21 strain to express them. Later, we used several methods to identify the target peptides in the extracellular fluid, the outer environment. Those methods include the dot-immunoblotting, ELISA and Western blotting. Those methods’ detail description are as below and the results are being discussed in detail.