Team:Goettingen/week7-2

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Revision as of 09:48, 22 September 2012

Deutsch  / English 

#2 Speed Improvement - 7th week

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V06_11


V06_11_1 Miniprep of the new flhDC-promoter constructs
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformation of the new flhDC-promoter constructs into E. coli (DH10B)
  • Experiment:
    For the chemical retransformation the standard protocol was followed. The following constructs were transformed into E. coli:

    20G - #1
    20G - #2
    2G - #1
    2G - #2
    2G - #3
    20I - #2
    20I - #3
    18O - #2
    18O - #3
    18K - #1
    18K - #2
    18K - #3

  • Observations & Results:
    The retransformation was successful since all plates showed numeours colonies except the negative control.


V06_12


Preparation of over night cultures
  • Experiment:
    In order to gain further plasmid material over night cultures of one colonie of all eight flhDC-promoter constructs were prepared for subsequent plasmid isolation.

    - 20E - #2
    - 20G - #1
    - 2G - #1
    - 20I - #2
    - 18M - #2
    - 18O - #2
    - 18K - #1
    - 18C - #2



V06_13


V06_13_1 Miniprep of the new flhDC-promoter constructs
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_13_2 Chemical transformation of pBAD-sfGFP into E. coli (DH10B)
  • Experiment:
    For the chemical transformation the standard protocol was followed.
  • Observations & Results:
    The transformation was successful since all plates showed numeours colonies except the negative control.


V06_14


Preparation of over night cultures
  • Experiment:
    Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.


V06_15


Miniprep of pBAD-sfGFP
  • Experiment:
    Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.


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