Team:Goettingen/week2-2

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<li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
<li>Experiment: <br>  The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual. </li>
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<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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Revision as of 09:38, 22 September 2012

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#2 Speed Improvement - 2nd week

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V05_08


Amplification of flhDC gene
  • Experiment:
    Using the isolated genomic DNA of E. coli (DH10B) as template, the gene flhDC was amplified by pfu polymerase according to the following protocol.



V05_10


Separation of flhDC
  • Experiment:
    The amplicon was analysed and separated via application on an 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
  • Observations & Results:
    The gel featured a clear band at about 900 kb. The amplification of flhDC was successful.


V05_11


Purificaton of flhDC
  • Experiment:
    The PCR product was purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.


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