Team:Goettingen/week8-2

From 2012.igem.org

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<h2><b>V06_18 </b></h2><br>
<h2><b>V06_18 </b></h2><br>
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<b>Chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B)</i></b><br>
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<b>Chemical transformation of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B)</i></b><br>
<ul>
<ul>
<li>Experiment: <br>  
<li>Experiment: <br>  
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In order to gain further plasmid material of the new vector pSB1C3 was transformed into <i>E. coli</i> (DH10B) as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br>
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In order to gain further plasmid material of the new vector, pSB1C3 was transformed into <i>E. coli</i> (DH10B) as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br>
<li>Observations & Results: <br>
<li>Observations & Results: <br>
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The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li>
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The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotic concentration in the agar was simply to high.</li>
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<ul>
<ul>
<li>Experiment: <br>
<li>Experiment: <br>
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Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li>
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pSB1C3 was transformed into <i>E. coli</i> (DH10B) as described in the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. </li>
<li>Observations & Results: <br>
<li>Observations & Results: <br>
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The transformation was successful.Due to the possesion of a gene encoding for <i>RFP</i> the developed colonied featured a red color. On the negative control no colonies were observed.
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The transformation was successful.Due to the possession of a gene encoding for <i>RFP</i> the developed colonies featured a red color. On the negative control no colonies were observed.
</li></ul>
</li></ul>
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<ul>
<ul>
<li>Experiment:  <br>
<li>Experiment:  <br>
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  Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br>  
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  Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only derogation from the standard transformation <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. <br>  
<li>Observations & Results: <br>
<li>Observations & Results: <br>
The transformation was again not successful. Only the positive control showed any growth.  </li>
The transformation was again not successful. Only the positive control showed any growth.  </li>
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18M-<i>tar</i> <br>
18M-<i>tar</i> <br>
19C-<i>tar</i> <br>
19C-<i>tar</i> <br>
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<br></td></tr>
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</table>
</table>

Revision as of 09:01, 22 September 2012

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