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Team:Bielefeld-Germany/Labjournal/week3
From 2012.igem.org
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| + | * '''Team Activity Tests''': Our ''T.versicolor'' laccase and the ABTS arrived! We couldn´t wait to start, so we set up the stock solutions we will need, such as sodium acetat buffer (pH 5), 10 mM ABTS and deluted laccase. | ||
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===Saturday May 19th=== | ===Saturday May 19th=== | ||
===Sunday May 20th=== | ===Sunday May 20th=== | ||
Revision as of 13:53, 18 September 2012
Contents |
Week 3 (05/14 - 05/20/12)
weekly seminar:
- first lab service: Robert
- our GFP, which we wanted to use for the summer school for pupils, does not work
- first competent cells have to be made: Julia S. and Robert
- Decision to buy a commercial laccase to establish the analytics and the enzyme tests
- our expose has to be translated into english: Malak
- last planning for our waver sell
- Julia S. is creating a vector for Pichia pastoris and is now looking for sequences
- The [http://www.bmbf.de/en/index.php BMBF] invites all german iGEM teams to Berlin to attend at the Biotechnologie2020+ strategy process
Monday May 14th
Tuesday May 15th
- Team Activity Tests: For some pre test and characterization for our future laccase activity standard we ordered [http://www.sigmaaldrich.com/catalog/product/sigma/53739?lang=de®ion=DE laccase] from Trametes versicolor. As well we had to order a substrate that the laccase could use to demonstrate its abilities. According to the literature [http://www.sigmaaldrich.com/catalog/product/sigma/a1888?lang=de®ion=DE ABTS] is a well working substrate to characterize oxidizing enzym activity. So we ordered.
Wednesday May 16th
- Team Bacterial Laccases: For cloning our laccases we need pSB1C3 backbone. Therefore we we transformed [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] (pSB1C3 with RFP) in competent KRX cells.
Thursday May 17th
- Team Modeling: Meeting Mrs. Lutter, a mathematics prof. of our course of studies and looking for our first model of a metabolic pathway, finding out, that we don't need such a complex model. Start thinking that we want and what we need.
- Team Bacterial Laccases: Plasmid isolation of BBa_J04450 (pSB1C3 + RFP).
Friday May 18th
- Team Activity Tests: Our T.versicolor laccase and the ABTS arrived! We couldn´t wait to start, so we set up the stock solutions we will need, such as sodium acetat buffer (pH 5), 10 mM ABTS and deluted laccase.
Saturday May 19th
Sunday May 20th
Sunday
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