Team:Goettingen/week2-3

(Difference between revisions)

Revision as of 19:17, 17 September 2012

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Amplification of tar gene

Experiment:
PCR was performed over night with the Tar forward and reverse primers using Pfu polymerase and the isolated genomic DNA from DH10B as template according to protocol.

Amplification of tar gene

Experiment:
PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
Observations & Results:
A band at about 1.6 kb was detected. Amplification of tar was successful.

Amplification of tar gene

Experiment:
PCR clean-up using peqGOLD Gel Extraction Kit according to the manual.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 538: / Line 538: @@
 <b>Amplification of <i>tar</i> gene</b><br>
 <ul>
-<li>Experiment: <br>PCR was performed with isolated genomic DNA from DH10B, forward and reverse primers of <i>tar</i> and using <i>Pfu</i> polymerase according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods"> protocol</a>.
+<li>Experiment: <br>PCR was performed over night with the Tar forward and reverse primers using <i>Pfu</i> polymerase
+and the isolated genomic DNA from DH10B as template according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods"> protocol</a>.
 </li>
 </ul>
@@ Line 550: / Line 551: @@
 <b>Amplification of <i>tar</i> gene</b><br>
 <ul>
-<li>Experiment: <br>PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used. A band at about 1.6 kb was detected. Amplification of <i>tar</i> was successful.
+<li>Experiment: <br>PCR was controlled by running a 1% agarose gel. As standard, Gene Ruler 1kb ladder (ThermoScientific) was used.
-</li>
+<br>
+<li>Observations & Results: <br>
+A band at about 1.6 kb was detected. Amplification of <i>tar</i> was successful.
 </ul>
 <br></td></tr>