Team:Goettingen/week8-2

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#2 Speed Improvement - 8th week

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V06_18

Chemical transformartion of the BioBrick plasmid pSB1C3 into E. coli(DH10B)

Experiment:
In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol.
Observations & Results:
The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.

V06_19

V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into E. coli(DH10B)

Experiment:
The transformation was conducted as described in the protocol, however, a lower chloramphenicol concentration was used.
Observations & Results:
The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.

V06_19_2 Chemical transformartion of pBAD-sfGFP into E. coli (DH10B)

Experiment:
For the chemical transformation the standard protocol was followed.
Observations & Results:
The transformation was successful since all plates showed numeours colonoes except the negative control.

V06_14

Preparation of over night cultures

Experiment:
Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.

V06_15

Miniprep of pBAD-sfGFP

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

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@@ Line 536: / Line 536: @@
 In order to gain further plasmid material of the new vector pSB1C3 was transformed into <i>E. coli</i>(DH10B) as described in the protocol. <br>
 <li>Observations & Results: <br>
-The transformation was not successful. No colonies could be observed at the plates. Since this vector has a canamycin resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li>
+The transformation was not successful. No colonies could be observed at the plates. Since this vector has a chloramphenicol resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li>
 </table>
@@ Line 544: / Line 544: @@
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b>V06_13 </b></h2><br>
+<h2><b>V06_19 </b></h2><br>
-<b>V06_13_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
+<b>V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i>(DH10B) </b><br>
 <ul>
 <li>Experiment:  <br>
-Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
+The transformation was conducted as described in the protocol, however, a lower chloramphenicol concentration was used.<br>
-<li>
+<li>Observations & Results: <br>
+The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.
+</li>
 <br>
-<b>V06_013_2 Chemical transformartion of pBAD-<i>sfGFP</i> into E. coli (DH10B)</b><br>
+<b>V06_19_2 Chemical transformartion of pBAD-<i>sfGFP</i> into E. coli (DH10B)</b><br>
 <ul>
 <li>Experiment:  <br>