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From 2012.igem.org

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-	<h2><b>V06_11 </b></h2><br>	+	<h2><b>V06_18 </b></h2><br>
-	<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>	+	<b>Chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i>(DH10B)</i></b><br>
	<ul>		<ul>
-	<li>Experiment: <br>	+	<li>Experiment: <br>
-	Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.	+	In order to gain further plasmid material of the new vector pSB1C3 was transformed into <i>E. coli</i>(DH10B) as described in the protocol. <br>
-		+
-	</ul>	+
-	<br>	+
-	<b>V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)</b><br>	+
-	<ul>	+
-	<li>Experiment:  <br>	+
-	For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:<br>	+
-	20G - #1 <br>	+
-	20G - #2 <br>	+
-	2G - #1 <br>	+
-	2G - #2 <br>	+
-	2G - #3 <br>	+
-	20I - #2 <br>	+
-	20I - #3 <br>	+
-	18O - #2 <br>	+
-	18O - #3 <br>	+
-	18K - #1 <br>	+
-	18K - #2 <br>	+
-	18K - #3 <br></li>	+
	<li>Observations & Results: <br>		<li>Observations & Results: <br>
-	The retransformation was successful since all plates showed numeours colonoes except the negative control.	+	The transformation was not successful. No colonies could be observed at the plates. Since this vector has a canamycin resistance instead of ampicillin as the usually used pUC18 we suggest that the antibiotics concentration in the agar was simply to high.</li>
-	</ul>	+
-	<br></td></tr>	+
-	</table>	+
-		+
-	<br>	+
-		+
-	<table cellpadding="20 px" border="1" bordercolor="black" valign="top">	+
-	<tr bordercolor="black" valign="top">	+
-	<td width="900" bordercolor="black" valign="top">	+
-	<h2><b>V06_12 </b></h2><br>	+
-	<b>Preparation of over night cultures</i></b><br>	+
-	<ul>	+
-	<li>Experiment: <br>	+
-	In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>	+
-	20E - #2 <br>	+
-	20G - #1 <br>	+
-	2G - #1 <br>	+
-	20I - #2 <br>	+
-	18M - #2 <br>	+
-	18O - #2 <br>	+
-	18K - #1 <br>	+
-	18C - #2 <br>	+
-	<br></td></tr>	+
	</table>		</table>

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Revision as of 10:52, 14 September 2012