Team:Goettingen/week8-2

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#2 Speed Improvement - 8th week

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V06_11

V06_11_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)

Experiment:
For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:
20G - #1
20G - #2
2G - #1
2G - #2
2G - #3
20I - #2
20I - #3
18O - #2
18O - #3
18K - #1
18K - #2
18K - #3
Observations & Results:
The retransformation was successful since all plates showed numeours colonoes except the negative control.

V06_12

Preparation of over night cultures

Experiment:
In order to gain further plasmid material over night cultures of one colonie of all eight flhDC-promoter constructs were prepared for subsequent plasmid isolation.
20E - #2
20G - #1
2G - #1
20I - #2
18M - #2
18O - #2
18K - #1
18C - #2

V06_13

V06_13_1 Miniprep of the new flhDC-promoter constructs

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
V06_013_2 Chemical transformartion of pBAD-sfGFP into E. coli (DH10B)
- Experiment:
  For the chemical transformation the standard protocol was followed.
- Observations & Results:
  The transformation was successful since all plates showed numeours colonoes except the negative control.

V06_14

Preparation of over night cultures

Experiment:
Over night cultures of the pBAD-sfGFP clones were prepared in order to isolate the plasmids.

V06_15

Miniprep of pBAD-sfGFP

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.

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Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 513: / Line 513: @@
 Language: <img height="20", src="http://www.patrickreinke.de/igem/eng.jpg"> English, <img height="20", src="http://www.patrickreinke.de/igem/deu.jpg"> <a href="https://2012.igem.org/Team:Goettingen/main_deu">Deutsch</a> <br>
 <br>
-<table id="toc" class="toc"><tbody><tr><td><div id="toctitle"><h2>Contents</h2> <span class="toctoggle">[<a href="javascript:toggleToc()" class="internal" id="togglelink">hide</a>]</span></div>
-<ul>
-<li class="toclevel-1"><a href="#week8"><span class="tocnumber">1</span> <span class="toctext">8th week (18th to 24th June)</span></a></li>
-<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week8#1"><span class="tocnumber"></span> <span class="toctext">#1 Selection / Swimming</span></a></li>
-<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week8#2"><span class="tocnumber"></span> <span class="toctext">#2 Speed Improvement</span></a></li>
-<li class="toclevel-1"> <div style="text-indent:10px;"><a href="#week8#3"><span class="tocnumber"></span> <span class="toctext">#3 Chemoreceptor Library</span></a></li>
-</ul>
-</td></tr></tbody></table>
-<br>
@@ Line 537: / Line 520: @@
 <td style="padding: 0pt 20px 0pt 0pt;" width="900px">
 <font face="Verdana" size="-1">
-<h2><b><a name="week8#1">#1 Selection / Swimming</a></b></h2>
+<h2><b><a name="week8#2">#2 Speed Improvement - 8th week</a></b></h2>
 <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
 <br>
 <p align="justify" style="line-height:1.6em">
-Under process..
+</p>
+<p align="justify" style="line-height:1.6em">
 </p>
 <table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b></b></h2><br>
+<h2><b>V06_11 </b></h2><br>
-<b></b><br>
+<b>V06_11_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
 <ul>
-<li>Experiment:  <br> </li>
+<li>Experiment:  <br>
-<li>Description: <br> </li>
+Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
-<li>Observations & Results: <br> </li>
 </ul>
 <br>
-<b><i/></b><br>
+<b>V06_11_2 Chemical retransformartion of the new flhDC-promoter constructs into E. coli (DH10B)</b><br>
 <ul>
-<li>Experiment: </li>
+<li>Experiment:  <br>
-<li>Description: <br> </li>
+For the chemical retransformation the standard protocol for transformation was followed. The following constructs were transformed into E. coli:<br>
-<li>Observations & Results: <br> </li>
+G - #1 <br>
+G - #2 <br>
+G - #1 <br>
+G - #2 <br>
+G - #3 <br>
+I - #2 <br>
+I - #3 <br>
+O - #2 <br>
+O - #3 <br>
+K - #1 <br>
+K - #2 <br>
+K - #3 <br></li>
+<li>Observations & Results: <br>
+The retransformation was successful since all plates showed numeours colonoes except the negative control.
 </ul>
 <br></td></tr>
 </table>
-<br>
-<br>
 <br>
+<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
+<tr bordercolor="black" valign="top">
+<td width="900" bordercolor="black" valign="top">
+<h2><b>V06_12 </b></h2><br>
+<b>Preparation of over night cultures</i></b><br>
+<ul>
+<li>Experiment: <br>
+In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br>
+E - #2 <br>
+G - #1 <br>
+G - #1 <br>
+I - #2 <br>
+M - #2 <br>
+O - #2 <br>
+K - #1 <br>
+C - #2 <br>
+<br></td></tr>
+</table>
 <br>
-<font face="Verdana" size="-1">
-<h2><b><a name="week8#2">#2 Speed Improvement</a></b></h2>
-<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
-<br>
-<p align="justify" style="line-height:1.6em">
-Under process...
-</p>
 <table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b></b></h2><br>
+<h2><b>V06_13 </b></h2><br>
-<b></b><br>
+<b>V06_13_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br>
 <ul>
-<li>Experiment:  <br> </li>
+<li>Experiment:  <br>
-<li>Description: <br> </li>
+Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
-<li>Observations & Results: <br> </li>
+<li>
-</ul>
 <br>
-<b><i/></b><br>
+<b>V06_013_2 Chemical transformartion of pBAD-<i>sfGFP</i> into E. coli (DH10B)</b><br>
 <ul>
-<li>Experiment: </li>
+<li>Experiment:  <br>
-<li>Description: <br> </li>
+For the chemical transformation the standard protocol was followed. <br>
-<li>Observations & Results: <br> </li>
+<li>Observations & Results: <br>
-</ul>
+The transformation was successful since all plates showed numeours colonoes except the negative control.</li>
 <br></td></tr>
 </table>
-<br>
-<br>
 <br>
-<font face="Verdana" size="-1">
-<h2><b><a name="week8#3">#3 Chemoreceptor Library</a></b></h2>
-<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
-<br>
-<p align="justify" style="line-height:1.6em">
-Under process...
-</p>
 <table cellpadding="20 px" border="1" bordercolor="black" valign="top">
 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b></b></h2><br>
+<h2><b>V06_14 </b></h2><br>
-<b></b><br>
+<b> Preparation of over night cultures</i></i></b><br>
 <ul>
-<li>Experiment:  <br> </li>
+<li>Experiment: <br>
-<li>Description: <br> </li>
+Over night cultures of the pBAD-<i>sfGFP</i> clones were prepared in order to isolate the plasmids. <br>
-<li>Observations & Results: <br> </li>
-</ul>
-<br>
-<b><i/></b><br>
-<ul>
-<li>Experiment: </li>
-<li>Description: <br> </li>
-<li>Observations & Results: <br> </li>
-</ul>
 <br></td></tr>
 </table>
-<br>
-<br>
-<br>
-<br>
 <br>
+<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
+<tr bordercolor="black" valign="top">
+<td width="900" bordercolor="black" valign="top">
+<h2><b>V06_15 </b></h2><br>
+<b> Miniprep of pBAD-<i>sfGFP</i></i></i></b><br>
+<ul>
+<li>Experiment: <br>
+Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. <br>
+<br></td></tr>
+</table>
-<br>
 <br>
 <body id="top">