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Team:TU Darmstadt/Materials/tctB197
From 2012.igem.org
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rev: gtttcttc'''ctgcagcggccgctactagta'''ttacagccaccccgtttcagtcag | rev: gtttcttc'''ctgcagcggccgctactagta'''ttacagccaccccgtttcagtcag | ||
=== tctB197 RBS === | === tctB197 RBS === | ||
| - | [https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the insertion of the arabinose promotor RBS site. | + | [https://2012.igem.org/Team:TU_Darmstadt/Materials#Primers Primers] for the insertion of the arabinose promotor RBS site based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101]. |
5' 3' | 5' 3' | ||
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAA AGAGGGGAC AAtactagatgtctgattccttccaagatctggc | fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAA AGAGGGGAC AAtactagatgtctgattccttccaagatctggc | ||
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttacagccaccccgtttcagtcag | rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttacagccaccccgtttcagtcag | ||
Revision as of 00:46, 14 September 2012
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB197]
Primers for the construction of a BioBrick containing the small subunit B2 of the tripartite tricarboxylate transporter family.
tctB197 BioBrick
5' BioBrick Annealing 3' fwd: gtttcttcgaattcgcggccgcttctagatgtctgattccttccaagatctggc rev: gtttcttcctgcagcggccgctactagtattacagccaccccgtttcagtcag
tctB197 RBS
Primers for the insertion of the arabinose promotor RBS site based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101].
5' 3' fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAA AGAGGGGAC AAtactagatgtctgattccttccaagatctggc rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttacagccaccccgtttcagtcag
