Team:TU Darmstadt/Materials/tctB197

tctB197

Primers for the construction of a BioBrick containing the small subunit B2 of the tripartite tricarboxylate transporter family.

tctB197 BioBrick

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgtctgattccttccaagatctggc
rev: gtttcttcctgcagcggccgctactagtattacagccaccccgtttcagtcag

tctB197 RBS

Primers for the insertion of the arabinose promotor RBS site based on the sequence of BBa_J61100 and BBa_J61101.

     5'                                                                           3'
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAA AGAGGGGAC AAtactagatgtctgattccttccaagatctggc
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttacagccaccccgtttcagtcag