Team:TU Darmstadt/Materials/tctB197

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Revision as of 00:35, 14 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808005 tctB197]

Primers for the construction of a BioBrick containing the small subunit B2 of the tripartite tricarboxylate transporter family.

tctB197 BioBrick 

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgtctgattccttccaagatctggc
rev: gtttcttcctgcagcggccgctactagtattacagccaccccgtttcagtcag

tctB197 RBS

Primers for the insertion of the arabinose promotor RBS site. 

     5'                                                                           3'
fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAA AGAGGGGAC AAtactagatgtctgattccttccaagatctggc
rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttacagccaccccgtttcagtcag
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