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Team:TMU-Tokyo/Project device2
From 2012.igem.org
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| - | <i>Escherichia coli</i> has original formaldehyde dehydrogenase, but it depends on glutathione | + | <i>Escherichia coli</i> has original formaldehyde dehydrogenase(FDH), but it depends on glutathione existence. Instead of it, we found glutathione-independent FDH in <i>Pseudomonas putida</i>. Formaldehyde dehydrogenase changes formaldehyde to formate.(HCHO → HCOOH)<Br> |
| + | <Br> | ||
| + | The reason we didn't select <i>Escherichia coli</i>'s own FDH is that glutathione-independent one can act in much more situations. For example, if Cheff Ant E.coli dies, <i>Escherichia coli</i>'s own FDH loses its substrate because of glutathione absence.<Br> | ||
| + | <Br> | ||
| + | This device is composed of its gene and constitutive promoter.<Br> | ||
<Br> | <Br> | ||
</p> | </p> | ||
Revision as of 20:01, 12 September 2012
Device2;
Producing formaldehyde dehydrogenase, changing formaldehyde to formate
Escherichia coli has original formaldehyde dehydrogenase(FDH), but it depends on glutathione existence. Instead of it, we found glutathione-independent FDH in Pseudomonas putida. Formaldehyde dehydrogenase changes formaldehyde to formate.(HCHO → HCOOH)
The reason we didn't select Escherichia coli's own FDH is that glutathione-independent one can act in much more situations. For example, if Cheff Ant E.coli dies, Escherichia coli's own FDH loses its substrate because of glutathione absence.
This device is composed of its gene and constitutive promoter.
