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Team:TMU-Tokyo/Project device1
From 2012.igem.org
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| - | In <i>frmRAB</i> operon, which origin is chromosome of <i>Escherichia coli</i>, <i>frmAB</i> is normally repressed by frmR. But, when formaldehyde exists, <i>frmR</i> loses its inhibitory and <i>frmAB</i> is expressed(2). This device is composed of <i>frmR</i> gene, its promoter and GFP gene.</p> | + | In <i>frmRAB</i> operon, which origin is chromosome of <i>Escherichia coli</i>, <i>frmAB</i> is normally repressed by frmR. But, when formaldehyde exists, <i>frmR</i> loses its inhibitory and <i>frmAB</i> is expressed. Repressing activity of frmR is shown quantitatively.(2)<Br> |
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| + | <Center><Img Src="https://static.igem.org/mediawiki/2012/6/6c/FrmR.jpg" Width="65%" Height="65%"></Center> | ||
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| + | This device is composed of <i>frmR</i> gene, its promoter and GFP gene.</p> | ||
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<center><Img Src="https://static.igem.org/mediawiki/2012/3/3a/Device1.png"></center><Br> | <center><Img Src="https://static.igem.org/mediawiki/2012/3/3a/Device1.png"></center><Br> | ||
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2. <Strong>Christopher D. Herring and Frederick R. Blattner.</Strong> 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator <i>frmR</i> [<A Href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC522192/">PubMed</A>]<Br> | 2. <Strong>Christopher D. Herring and Frederick R. Blattner.</Strong> 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator <i>frmR</i> [<A Href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC522192/">PubMed</A>]<Br> | ||
<Br> | <Br> | ||
| - | fig1. a) When formaldehyde doesn't exist, <i>frmR</i> represses upstream promoter. In this state, transcription is stopped and GFP isn't produced. b) When formaldehyde exists, it makes <i>frmR</i> lose activity. Then promoter is released from <i>frmR</i>'s repress and begins to produce GFP.<Br> | + | fig1. In condition of frmR mass expression, frmAB expressing level was measured by RT-PCR.(2) This is its result. Compared with <Br> |
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| + | fig2. a) When formaldehyde doesn't exist, <i>frmR</i> represses upstream promoter. In this state, transcription is stopped and GFP isn't produced. b) When formaldehyde exists, it makes <i>frmR</i> lose activity. Then promoter is released from <i>frmR</i>'s repress and begins to produce GFP.<Br> | ||
</p> | </p> | ||
Revision as of 19:42, 12 September 2012
Device1;
Sensing formaldehyde and then producing GFP
In frmRAB operon, which origin is chromosome of Escherichia coli, frmAB is normally repressed by frmR. But, when formaldehyde exists, frmR loses its inhibitory and frmAB is expressed. Repressing activity of frmR is shown quantitatively.(2)
This device is composed of frmR gene, its promoter and GFP gene.
By using this device, we can visualize formaldehyde with GFP.
2. Christopher D. Herring and Frederick R. Blattner. 2004. Global Transcriptional Effects of a Suppressor tRNA and the Inactivation of the Regulator frmR [PubMed]
fig1. In condition of frmR mass expression, frmAB expressing level was measured by RT-PCR.(2) This is its result. Compared with
fig2. a) When formaldehyde doesn't exist, frmR represses upstream promoter. In this state, transcription is stopped and GFP isn't produced. b) When formaldehyde exists, it makes frmR lose activity. Then promoter is released from frmR's repress and begins to produce GFP.
