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Team:HokkaidoU Japan/Notebook/plastic Week 10
From 2012.igem.org
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| + | ==Colony PCR== | ||
| + | <p> | ||
| + | We confirmed the length of constructs, [PhaB on pSB1C3] and [RBS-PhaC-RBS-PhaA on pSB1A2] by colony PCR.<br />The result showed ligation to take PhaB on pSB1C3 went well.<br />But ligation [RBS-PhaC on pSB1A2] with [RBS-PhaA] failed. | ||
| + | </p> | ||
| - | + | ==Liquid culture== | |
| - | + | <p> | |
| - | + | We add 2ml LB and 2ul antibiotic to ideal colony suspension and begun to cultivate at 37C, 180rpm. | |
| + | </p> | ||
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Revision as of 12:54, 3 September 2012
Contents |
September 3rd
Single colony isolation
The colony of pGEM PhaCAB in JM109 was isolated to plate1 and plate2.
The colony of pGEM PhaCAB in DH5a was isolated to plate3.
Plate 1~3 was started to incubate from 18:00.
Colony PCR
We confirmed the length of constructs, [PhaB on pSB1C3] and [RBS-PhaC-RBS-PhaA on pSB1A2] by colony PCR.
The result showed ligation to take PhaB on pSB1C3 went well.
But ligation [RBS-PhaC on pSB1A2] with [RBS-PhaA] failed.
Liquid culture
We add 2ml LB and 2ul antibiotic to ideal colony suspension and begun to cultivate at 37C, 180rpm.
