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Team:Bielefeld-Germany/Labjournal/week7
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* '''Team Activity Tests''': Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U ''T. versicolor'' laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase does not seem to be totally satisfied at 4°C. I would not either. | * '''Team Activity Tests''': Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U ''T. versicolor'' laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase does not seem to be totally satisfied at 4°C. I would not either. | ||
| - | * '''Team Bacterial laccase''': Because our PCRs have not worked well we thought it may depend on the primer annealing temperature so we did gradient PCR with the same conductions as before ([https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 PCR June 4th]) . But this also showed as no result. | + | * '''Team Bacterial laccase''': Because our PCRs have not worked well we thought it may depend on the primer annealing temperature so we did gradient PCR with the same conductions as before ([https://2012.igem.org/Team:Bielefeld-Germany/Labjournal/week6 PCR June 4th]) . But this also showed as no result. Our next idea was to let the bacteria grow in media and isolate genomic DNA. |
===Friday June 15th=== | ===Friday June 15th=== | ||
===Saturday June 16th=== | ===Saturday June 16th=== | ||
===Sunday June 17th=== | ===Sunday June 17th=== | ||
Revision as of 09:50, 3 September 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 7 (06/11 - 06/17/12)
Monday June 11th
- Team Sequencing: Sequencing of the first finished biobricks.
Tuesday June 12th
Wednesday June 13th
- Team Modeling: programming our first differential equation and finding the ODE15s function witch solves these equations.
- Team Bacterial laccase: since the GC amount of the S. griseus and S. lavendulae laccases are high we used betain to solve the PCR problem.
-> Addition of betain did not changed anything from the result we still haven't had our laccase DNA.
Thursday June 14th
- Team Activity Tests: Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U T. versicolor laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase does not seem to be totally satisfied at 4°C. I would not either.
- Team Bacterial laccase: Because our PCRs have not worked well we thought it may depend on the primer annealing temperature so we did gradient PCR with the same conductions as before (PCR June 4th) . But this also showed as no result. Our next idea was to let the bacteria grow in media and isolate genomic DNA.
