Team:TMU-Tokyo

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Our team project　Chef Ant E.coli

Project Abstract

Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.(1) We planed to create E.coli with an ability to　detect and detoxify formaldehyde., named Chef Ant E.coli. About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP. We plan to overexpress　two enzymes in E.coli. First,formaldehyde dehydrogenase decomposes formaldehyde to formic acid. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida. Second,formic acid dehydrogenase converts formic acid to CO2 and H2O. The gene of formic acid dehydrogenase is from Methylobacterium extorquens.

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-Our project's goal is creating E.coli to resolve formaldehyde.
+Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since fromaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. These days, in Japan, the detection of formaldehyde in Tone river became an issue.(1)
-Formaldehyde is a toxic substance that exists close around our lives (cf. agricultural chemicals, antiseptics in hospital, paint of building materials, etc.) and effects negatively on human body even at a relatively low concentration. Moreover, mass production of formaldehyde is done at many factories these days.  So it could easily happen that formaldehyde flows out environment over its permissible amount. In fact, an over limit concentration of formaldehyde was detected from Tone River and became a hot topic in Japan (May, 2012).In these points, we make a goal to create E.coli with ability to detoxification. E.coli has a small amount of formaldehyde dehydrogenase. And this enzyme change formaldehyde to formic acid. E.coli has an enzyme too to change formic acid to hydrogen and carbon dioxide. Thesefore, we plan to overexpress these two enzymes in E.coli. The degrading process by the 2 enzymes follows the formula below.
+We planed to create E.coli with an ability to　detect and detoxify formaldehyde., named Chef Ant E.coli.
+About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP.
-<p class="description" style="font-size: 15pt">Formaldehyde  HCHO -> Formic acid HCOOH -> CO2 + H2O </p>
+We plan to overexpress　two enzymes in E.coli.
+First,formaldehyde dehydrogenase decomposes formaldehyde to formic acid. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida.
+Second,formic acid dehydrogenase converts formic acid to CO2 and H2O. The gene of formic acid dehydrogenase is from Methylobacterium extorquens.
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-<font size="3" >There are several reasons why we use E.coli for detoxifying formaldehyde. First, cultivating E. coli can easily do mass production of the degrading enzymes. Second, this system can be industrially applied. For example, if an Apotosis function is attached to an E. coli that detoxifies formaldehyde, it can be used to purify particular contaminated environments and also tap water. Moreover, hydrogen is obtained as a degradation product of formic acid. Since hydrogen is expected as a new clean energy for fuel cell, it will also be a great industrial development if its recovery becomes possible from our formaldehyde degrading system by E. coli.</font>
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