"
Team:HokkaidoU Japan/Notebook/plastic Week 8
From 2012.igem.org
(Difference between revisions)
(→August 21th) |
(→Digestion) |
||
| Line 7: | Line 7: | ||
==August 20th== | ==August 20th== | ||
<div> | <div> | ||
| - | ==Digestion== | + | ===Digestion=== |
<p> | <p> | ||
A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br> | A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br> | ||
| Line 13: | Line 13: | ||
{|class="hokkaidou-table-digestion" | {|class="hokkaidou-table-digestion" | ||
|- | |- | ||
| - | |DNA solution PhaC | + | |DNA solution PhaC |
|12 ul | |12 ul | ||
|- | |- | ||
| Line 58: | Line 58: | ||
{|class="hokkaidou-table-digestion" | {|class="hokkaidou-table-digestion" | ||
|- | |- | ||
| - | |DNA solution | + | |DNA solution PhaB |
|7 ul | |7 ul | ||
|- | |- | ||
Revision as of 14:21, 26 August 2012
Contents |
August 20th
Digestion
A digestion to divide PhaC and pSB1C3 with XbaI and SpeI. </ br>
| DNA solution PhaC | 12 ul |
| XbaI | 1 ul |
| SpeI | 1 ul |
| 10xM buffer | 2 ul |
| DW | 4 ul |
| Total | 20 ul |
A digestion to divide PhaA and XbaI site and SpeI site.</ br>
| DNA solution PhaA | 7 ul |
| XbaI | 1 ul |
| SpeI | 1 ul |
| 10xM buffer | 2 ul |
| DW | 9 ul |
| Total | 20 ul |
A digestion to divide PhaB and XbaI site and SpeI site.</ br>
| DNA solution PhaB | 7 ul |
| XbaI | 1 ul |
| SpeI | 1 ul |
| 10xM buffer | 2 ul |
| DW | 9 ul |
| Total | 20 ul |
August 21th
Elecrophoresis
Gel extraction
Gel ectraction of PhaA, PhaB, pSB1C3 digestion result.</ br> We used FastGene Gel&PCR extraction kit(NipponGenetics)and got 50 ul of DNA solution.</ br>
August 22th
Colony PCR
August 23th
Colony PCR
August 24th
Colony PCR
August 25th
Colony PCR
August 26th
Mini prep of DNA
