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Team:HokkaidoU Japan/Notebook/plastic Week 8
From 2012.igem.org
(Difference between revisions)
(→August 20th) |
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==August 20th== | ==August 20th== | ||
<div> | <div> | ||
| - | + | ||
<p> | <p> | ||
| + | A digestion to divide PhaC and pSB1C3 using . | ||
| + | |||
| + | {|class="hokkaidou-table-digestion" | ||
| + | |- | ||
| + | |DNA solution PhaC ( 257ng/ul) | ||
| + | |12 ul | ||
| + | |- | ||
| + | |XbaI | ||
| + | |1 ul | ||
| + | |- | ||
| + | |SpeI | ||
| + | |1 ul | ||
| + | |- | ||
| + | |10xM buffer | ||
| + | |2 ul | ||
| + | |- | ||
| + | |DW | ||
| + | |4 ul | ||
| + | |- | ||
| + | |Total | ||
| + | |20 ul | ||
| + | |} | ||
| + | |||
</p> | </p> | ||
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<div class="hokkaidou-notebook-daily"> | <div class="hokkaidou-notebook-daily"> | ||
| + | |||
==August 21th== | ==August 21th== | ||
<div> | <div> | ||
Revision as of 12:36, 26 August 2012
Contents |
August 20th
A digestion to divide PhaC and pSB1C3 using .
| DNA solution PhaC ( 257ng/ul) | 12 ul |
| XbaI | 1 ul |
| SpeI | 1 ul |
| 10xM buffer | 2 ul |
| DW | 4 ul |
| Total | 20 ul |
August 21th
Colony PCR
August 22th
Colony PCR
August 23th
Colony PCR
August 24th
Colony PCR
August 25th
Colony PCR
August 26th
Mini prep of DNA
