"

From 2012.igem.org

Revision as of 14:39, 23 August 2012 (view source) JSchirmacher (Talk | contribs) ← Older edit		Revision as of 14:51, 23 August 2012 (view source) JSchirmacher (Talk | contribs) Newer edit →
Line 6:		Line 6:
-	==Media==	+	==Media, buffer and other solutions==
		+	===Ampicillin stock solution===
		+	* Solubilize 100 mg mL<sup>-1</sup> Ampicillin
		+	* Store at -20 °C
		+
		+
		+	===Chloramphenicol stock solution===
		+	* Solubilize 20 mg mL<sup>-1</sup> Chloramphenicol in 100 % Ethanol
		+	* Store at -20 °C
		+
		+
		+	===TAE buffer===
		+	For 1 L of 50 x TAE buffer you need:
		+
		+	* 242.48 g Tris
		+	* 41.02 g Sodiumacetate
		+	* 18.612 g EDTA
		+	* Adjust pH to 7.8 with acetic acid
		+	* Solve in dH<sub>2</sub>O
		+
		+	10 mL of the stock is diluted in 1 L dH<sub>2</sub>O for the gel electrophoresis (0.5 x TAE buffer).
		+
		+
		+	===DNA loading buffer===
		+	* 50 % (v/v) glycerol
		+	* 1 mM EDTA
		+	* 0.1 % (w/v) bromphenol blue
		+	* Solve in ddH<sub>2</sub>O
		+
		+
		+	===LB media===
		+	For 1 L of LB media:
		+
		+	* 10 g Trypton
		+	* 5 g Yeast extract
		+	* 10 g NaCl
		+	* 12 g Agar-Agar (for plates)
		+	* Adjust pH to 7.4
		+
		+
		+	===YPD media===
		+	For 1 L of YPD media:
		+
		+	* 20 g Peptone
		+	* 10 g Yeast extract
		+	* 20 g Dextrose (add 50 mL sterile stock solution (40% dextrose))
		+	* Adjust pH to 6.5
		+
	==Primers==		==Primers==
	This is a list of primers we have used.		This is a list of primers we have used.

---

Revision as of 14:51, 23 August 2012

Contents 1 Materials 1.1 Media, buffer and other solutions 1.1.1 Ampicillin stock solution 1.1.2 Chloramphenicol stock solution 1.1.3 TAE buffer 1.1.4 DNA loading buffer 1.1.5 LB media 1.1.6 YPD media 1.2 Primers

Materials

This is where we are going to list all our materials, devices and equipment that we have used.

Media, buffer and other solutions

Ampicillin stock solution

• Solubilize 100 mg mL^-1 Ampicillin
• Store at -20 °C

Chloramphenicol stock solution

• Solubilize 20 mg mL^-1 Chloramphenicol in 100 % Ethanol
• Store at -20 °C

TAE buffer

For 1 L of 50 x TAE buffer you need:

• 242.48 g Tris
• 41.02 g Sodiumacetate
• 18.612 g EDTA
• Adjust pH to 7.8 with acetic acid
• Solve in dH₂O

10 mL of the stock is diluted in 1 L dH₂O for the gel electrophoresis (0.5 x TAE buffer).

DNA loading buffer

• 50 % (v/v) glycerol
• 1 mM EDTA
• 0.1 % (w/v) bromphenol blue
• Solve in ddH₂O

LB media

For 1 L of LB media:

• 10 g Trypton
• 5 g Yeast extract
• 10 g NaCl
• 12 g Agar-Agar (for plates)
• Adjust pH to 7.4

YPD media

For 1 L of YPD media:

• 20 g Peptone
• 10 g Yeast extract
• 20 g Dextrose (add 50 mL sterile stock solution (40% dextrose))
• Adjust pH to 6.5

Primers

This is a list of primers we have used.