"
Team:Bielefeld-Germany/Labjournal/week7
From 2012.igem.org
(Difference between revisions)
(→Wednesday June 13th) |
(→Wednesday June 13th) |
||
| Line 32: | Line 32: | ||
===Wednesday June 13th=== | ===Wednesday June 13th=== | ||
* '''Team Modeling''': programming our first differential equation and finding the ODE15s function witch solves these equations. | * '''Team Modeling''': programming our first differential equation and finding the ODE15s function witch solves these equations. | ||
| + | |||
| + | * '''Team Bacterial laccase''': since the GC amount of the ''S. griseus'' and ''S. lavendulae'' laccases are high we used betain to solve the PCR problem. | ||
| + | -> Addition of betain did not changed anything from the result we still haven't had our laccase DNA. | ||
===Thursday June 14th=== | ===Thursday June 14th=== | ||
Revision as of 09:39, 3 September 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 7 (06/11 - 06/17/12)
Monday June 11th
- Team Sequencing: Sequencing of the first finished biobricks.
Tuesday June 12th
Wednesday June 13th
- Team Modeling: programming our first differential equation and finding the ODE15s function witch solves these equations.
- Team Bacterial laccase: since the GC amount of the S. griseus and S. lavendulae laccases are high we used betain to solve the PCR problem.
-> Addition of betain did not changed anything from the result we still haven't had our laccase DNA.
Thursday June 14th
- Team Activity Tests: Since our Tecan microplate reader is not able to actively cool down to 4 °C we got the chance to meet the photometer Carry. Check "protocols" for further information about her. We used the same set up with 100 mM natrium acetate buffer, 0,1 U T. versicolor laccase and 0,1 mM ABTS as before but now measured at 4°C. Our team is planning to visit a municipal sewage plant for getting some insights into the water conditions there, so we will for sure test other temperatures after having more information. Let´s hope the water there is a little warmer since laccase doesn´t seem to be totally satisfied at 4°C. I wouldn´t either.
