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Team:HokkaidoU Japan/Notebook/plastic Week 6
From 2012.igem.org
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==DNA sequencing== | ==DNA sequencing== | ||
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| - | + | BBa_K342001(PhaC) was seqenced.<br /> | |
Revision as of 06:29, 30 August 2012
Contents |
August 6th
polymer extract
@Taguchi lab
- sample1 JM109 culture from July18th 1.4643g , 0.0054 (tube+polymer , polymer)
- sample2 JM109 culture from July18th 1.4642g , 0.0040
- sample3 DH5a 1.4666g , 0.0056
preculture
Inoculated from three different plates(Name it 1,2,3)
Cultured in media of LB 150microL+Amp1.5microL
30℃ 20:00-7th10:00
August 7th
liquid culture
We made 8sapmles to see if Panthotenic acid is nessesary for plastic growth.
Panthotenic acid (+)1.5ml 4samples
Panthotenic acid (+)0.7ml 2samples
Panthotenic acid (-)0.7ml 2samples
Started liquid culture from Aug 7th 11:00 until Aug 9th 09:50.</ br>
August 9th
@Taguchi lab
Harvest
We did the protocol of Harvest on 8samples.
August 10th
miniprep
We did the method of mini-prep for BBa_K342001(PhaC). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions.
August 11th
DNA sequencing
BBa_K342001(PhaC) was seqenced.
