"
Team:Bielefeld-Germany/Labjournal/week2
From 2012.igem.org
(Difference between revisions)
(→Labjournal) |
(→Week 2 (05/07 - 05/13/12)) |
||
| Line 24: | Line 24: | ||
==Week 2 (05/07 - 05/13/12)== | ==Week 2 (05/07 - 05/13/12)== | ||
| - | |||
* Primerdesign for isolation of laccases from genomic DNA of ''Xanthomonas campestris B100'', ''E. coli BL21(DE3)'' and ''Bacillus pumilus ATCC7061''. The forward primers were designed with T7 promotor-overhanging ends after prefix and the first 20 bases of the wanted gene. The reverse primers were designed with a HIS-Tag and tweo stop codons before suffix and the last 20 bases of the wanted gene without the stop codon. | * Primerdesign for isolation of laccases from genomic DNA of ''Xanthomonas campestris B100'', ''E. coli BL21(DE3)'' and ''Bacillus pumilus ATCC7061''. The forward primers were designed with T7 promotor-overhanging ends after prefix and the first 20 bases of the wanted gene. The reverse primers were designed with a HIS-Tag and tweo stop codons before suffix and the last 20 bases of the wanted gene without the stop codon. | ||
| Line 43: | Line 42: | ||
* After some empty agarose gels we finally isolated laccase gene CotA from ''Bacillus pumilus ATCC7061'' as a PCR product. As template we used the plasmid we got from the Swiss working group. | * After some empty agarose gels we finally isolated laccase gene CotA from ''Bacillus pumilus ATCC7061'' as a PCR product. As template we used the plasmid we got from the Swiss working group. | ||
* '''Team Modeling''': Looking for suitable Software and enzymkinetics to model the degradation of our substartes with the different laccases. Finding the Michaelis-Menten kinetics and matlab. | * '''Team Modeling''': Looking for suitable Software and enzymkinetics to model the degradation of our substartes with the different laccases. Finding the Michaelis-Menten kinetics and matlab. | ||
| + | === Monday May 7th === | ||
| + | * '''Team Student Academy:''' | ||
| + | === Tuesday May 8th === | ||
| + | === Wednesday May 9th === | ||
| + | === Thursday May 10th === | ||
| + | === Friday May 11th === | ||
Revision as of 16:06, 23 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 2 (05/07 - 05/13/12)
- Primerdesign for isolation of laccases from genomic DNA of Xanthomonas campestris B100, E. coli BL21(DE3) and Bacillus pumilus ATCC7061. The forward primers were designed with T7 promotor-overhanging ends after prefix and the first 20 bases of the wanted gene. The reverse primers were designed with a HIS-Tag and tweo stop codons before suffix and the last 20 bases of the wanted gene without the stop codon.
- Successful PCRs of laccase genes CopA from Xanthomonas campestris B100, CueO from E. coli BL21(DE3) with the isolated genomic DNA as template.
- Because we want to characterise laccases from different bacteria we had to order the bacterial strains, which weren't available at the University Bielefeld, from [http://www.dsmz.de/|DSMZ].
- [http://www.dsmz.de/catalogues/details/culture/DSM-7039.html?tx_dsmzresources_pi5%5BreturnPid%5D=304|Thermus thermophilus HB27]
- [http://www.dsmz.de/catalogues/details/culture/DSM-18197.html?tx_dsmzresources_pi5%5BreturnPid%5D=304|Bacillus halodurans C-125]
- [http://www.dsmz.de/catalogues/details/culture/DSM-40069.html?tx_dsmzresources_pi5%5BreturnPid%5D=304|Streptomyces lavendulae REN-7]
- [http://www.dsmz.de/catalogues/details/culture/DSM-40236.html?tx_dsmzresources_pi5%5BreturnPid%5D=304|Streptomyces griseus IFO 13350]
- After some empty agarose gels we finally isolated laccase gene CotA from Bacillus pumilus ATCC7061 as a PCR product. As template we used the plasmid we got from the Swiss working group.
- Team Modeling: Looking for suitable Software and enzymkinetics to model the degradation of our substartes with the different laccases. Finding the Michaelis-Menten kinetics and matlab.
Monday May 7th
- Team Student Academy:
