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Team:HokkaidoU Japan/Notebook/plastic Week 6
From 2012.igem.org
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30℃ 20:00-7th10:00<br /> | 30℃ 20:00-7th10:00<br /> | ||
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We did the method of mini-prep for BBa_K342001(PhaC). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. | We did the method of mini-prep for BBa_K342001(PhaC). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. | ||
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==August 11th== | ==August 11th== | ||
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<!-- DO NOT EDIT UNDER THIS LINE @iTakeshi --> | <!-- DO NOT EDIT UNDER THIS LINE @iTakeshi --> | ||
Revision as of 10:38, 11 August 2012
Contents |
August 6th
polymer extract
@Taguchi lab
- sample1 JM109 culture from July18th 1.4643g , 0.0054 (tube+polymer , polymer)
- sample2 JM109 culture from July18th 1.4642g , 0.0040
- sample3 DH5a 1.4666g , 0.0056
preculture
Inoculate from three different plates(Name it 1,2,3)
Culture in media of LB 150microL+Amp1.5microL
30℃ 20:00-7th10:00
August 10th
miniprep
We did the method of mini-prep for BBa_K342001(PhaC). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions.
August 11th
sequencing reaction
We took the sequence of phaC.
