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Team:University College London/Protocols/RestrictionEnzymeDigest1
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== Enzyme Digest == | == Enzyme Digest == | ||
</noinclude> | </noinclude> | ||
| - | 1 | + | '''Step 1 - Thawing cells:''' Thaw all materials on ice |
| - | 2 | + | '''Step 2 - Adding Ingredient:''' Add the following ingredients to autoclaved/sterile eppendorf tubes |
Insert Table | Insert Table | ||
| - | 3 | + | '''Step 3 - Addition of BioBrick:''' Flick contents gently and centrifuge. |
| - | 4 | + | '''Step 4 - Centrifuge:''' To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight |
| - | 5 | + | '''Step 5 - Digest Program:''' Then run on the following conditions (by turning the dial) |
RPM: 14000 | RPM: 14000 | ||
Revision as of 08:41, 1 August 2012
Enzyme Digest
Step 1 - Thawing cells: Thaw all materials on ice
Step 2 - Adding Ingredient: Add the following ingredients to autoclaved/sterile eppendorf tubes
Insert Table
Step 3 - Addition of BioBrick: Flick contents gently and centrifuge.
Step 4 - Centrifuge: To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight
Step 5 - Digest Program: Then run on the following conditions (by turning the dial)
RPM: 14000
Time: 1 minute
Temperature: 18oC
