Team:University College London/LabBook

From 2012.igem.org

(Difference between revisions)

Revision as of 11:39, 31 July 2012

Monday 23.7.12

Aim - Transformation of TetR BBa_C0040 BioBrick

(LOGO) Transformation Protocol 1

Step 1 – Thawing Cells: Use W3100 cell line created in Week 2 (Expt 2.1) Step 3 – Addition of BioBrick: To a 2ml eppendorf, add 1ul of the following BioBricks. Note: we have changed the protocol for our positive control. Previously it contained no BioBrick, but it has been recommended to us that we transform our positive control such that there is one for each BioBrick – this will tell us if the BioBrick has in any way affected cell viability. This will be used from this point onwards. Include an extra tube as a negative control, with no BioBrick added

Hello World

Sortable and collapsible table
Alphabetic	Numeric	Date	Unsortable
d	20	2008-11-24	This
b	8	2004-03-01	column
a	6	1979-07-23	cannot
c	4.2	1492-12-08	be
e	0	1601-08-13	sorted.

@@ Line 3: / Line 3: @@
 <img src="https://static.igem.org/mediawiki/2012/1/13/Ucl2012-labbook-graph7-1.png" />
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-==Lorem Ipsum ==
-test test test
+== Monday 23.7.12 ==
+'''Aim''' - Transformation of TetR BBa_C0040 BioBrick
+(LOGO) Transformation Protocol 1
+'''Step 1''' – Thawing Cells: Use W3100 cell line created in Week 2 (Expt 2.1)
+'''Step 3''' – Addition of BioBrick: To a  2ml eppendorf, add 1ul of the following BioBricks. Note: we have changed the protocol for our positive control. Previously it contained no BioBrick, but it has been recommended to us that we transform our positive control such that there is one for each BioBrick – this will tell us if the BioBrick has in any way affected cell viability. This will be used from this point onwards. Include an extra tube as a negative control, with no BioBrick added
 <html></div>
 <div class="experiment"></div>
 <img src="https://static.igem.org/mediawiki/2012/2/22/Ucl2012-labbook-graph7-2.png" /><div class="experimentContent">
 </html>
 == Hello World ==