"
Team:University College London/Protocols/Nanodrop
From 2012.igem.org
(Difference between revisions)
Rwilkinson (Talk | contribs) (Created page with "{{:Team:University_College_London/templates/head|coverpicture=week4}} == Nanodrop == Software ND-1000 Model: 1.Initialise the spectrophotometer by pipetting 1 µ of clean wat...") |
Rwilkinson (Talk | contribs) |
||
| Line 1: | Line 1: | ||
{{:Team:University_College_London/templates/head|coverpicture=week4}} | {{:Team:University_College_London/templates/head|coverpicture=week4}} | ||
| - | |||
== Nanodrop == | == Nanodrop == | ||
| Line 20: | Line 19: | ||
7.Once measurement complete, wipe surface | 7.Once measurement complete, wipe surface | ||
| + | |||
| + | {{:Team:University_College_London/templates/foot}} | ||
Revision as of 15:31, 16 July 2012
Nanodrop
Software ND-1000 Model:
1.Initialise the spectrophotometer by pipetting 1 µ of clean water onto lower optic surface, lowering the lever arm and selecting ‘initialise’ in the ND-1000 software
2.Wipe and add elution buffer as negative control. Click blank in ND-1000 software
3.Wipe and add 1 µl sample
4.On the software set lambda to 260nm
5.Lower the lever arm and click measure in ND-1000 software
6.Take readings for concentration and purity
7.Once measurement complete, wipe surface
