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Team:University College London/Protocols/RestrictionEnzymeDigest1
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1. Thaw all materials on ice | 1. Thaw all materials on ice | ||
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2. Add the following ingredients to autoclaved/sterile eppendorf tubes | 2. Add the following ingredients to autoclaved/sterile eppendorf tubes | ||
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3.Flick contents gently and centrifuge. | 3.Flick contents gently and centrifuge. | ||
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4.To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight | 4.To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight | ||
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5. Then run on the following conditions (by turning the dial) | 5. Then run on the following conditions (by turning the dial) | ||
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RPM: 14000 | RPM: 14000 | ||
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Time: 1 minute | Time: 1 minute | ||
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Temperature: 18oC | Temperature: 18oC | ||
{{:Team:University_College_London/templates/foot}} | {{:Team:University_College_London/templates/foot}} | ||
Revision as of 15:27, 16 July 2012
== Enzyme Digest ==
1. Thaw all materials on ice
2. Add the following ingredients to autoclaved/sterile eppendorf tubes
Insert Table
3.Flick contents gently and centrifuge.
4.To centrifuge, add sample eppendorf + balancer (fill another tube with equal volume of water if only have one sample) and screw lid on tight
5. Then run on the following conditions (by turning the dial)
RPM: 14000
Time: 1 minute
Temperature: 18oC
