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Team:HokkaidoU Japan/Notebook/Week 3
From 2012.igem.org
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==July 16th== | ==July 16th== | ||
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| + | ===Digestion=== | ||
| + | Results of digestion in 15th. | ||
| + | |||
| + | [[image:HokkaidoU2012 120716 Ag43 on pSB1C3 digestion with S&P-1.jpg|thumb|Digestion result image]] | ||
| + | Product:Ag43(K346007)=3120bp and 2070bp, pT7-RBS on pSB1K3=2247bp | ||
| + | We confirmed there are some kind of restriction enzyme site in K346007 (digested with SpeI, PstI) and pT7-RBS on pSB1K3 was successfully digested with EcoRI and PstI. Balance between d+(EcoRI) and d+(E&P:cut with EcoRI and PstI) is about 80bp. | ||
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Revision as of 06:55, 16 July 2012
July 16th
Digestion
Results of digestion in 15th.
Product:Ag43(K346007)=3120bp and 2070bp, pT7-RBS on pSB1K3=2247bp We confirmed there are some kind of restriction enzyme site in K346007 (digested with SpeI, PstI) and pT7-RBS on pSB1K3 was successfully digested with EcoRI and PstI. Balance between d+(EcoRI) and d+(E&P:cut with EcoRI and PstI) is about 80bp.
