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Team:Valencia Biocampus/Notebook
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== '''Notebook''' == | == '''Notebook''' == | ||
| + | '''16th July''': All the students have had a "wiki meeting" to plan how we want to design our wiki. The [[bacteria sub-team]]has set up a triple groove of bac1 vector transformed cells. | ||
'''13th July''': Today, the ['''bacteria sub-team'''] members did two mini-preps in order to get ''Bac1 construction'' purified. After that, they digested the plasmid which contained the construction of interest using the digestion enzymes ''EcoRI'' and ''PstI'' and they made an agarose gel where the samples were put. The picture obtained showed that it was perfect so the bacteria-members decided to plate using triple groove and let the cells divide. Furthemore, they glycerined the cultures with the tested constructions in order to have an stock of cell that the could use if necessary. | '''13th July''': Today, the ['''bacteria sub-team'''] members did two mini-preps in order to get ''Bac1 construction'' purified. After that, they digested the plasmid which contained the construction of interest using the digestion enzymes ''EcoRI'' and ''PstI'' and they made an agarose gel where the samples were put. The picture obtained showed that it was perfect so the bacteria-members decided to plate using triple groove and let the cells divide. Furthemore, they glycerined the cultures with the tested constructions in order to have an stock of cell that the could use if necessary. | ||
Revision as of 13:04, 16 July 2012
Notebook
16th July: All the students have had a "wiki meeting" to plan how we want to design our wiki. The bacteria sub-teamhas set up a triple groove of bac1 vector transformed cells.
13th July: Today, the [bacteria sub-team] members did two mini-preps in order to get Bac1 construction purified. After that, they digested the plasmid which contained the construction of interest using the digestion enzymes EcoRI and PstI and they made an agarose gel where the samples were put. The picture obtained showed that it was perfect so the bacteria-members decided to plate using triple groove and let the cells divide. Furthemore, they glycerined the cultures with the tested constructions in order to have an stock of cell that the could use if necessary.
12th July: Today, the bacterial cultures made the previous day grew succesfully, the [bacteria sub-team] members stringed several colonies and grew them in LB+Amp liquid media. In the afternoon, the last construction (Bac1) arrived so they transformed E.coli with it using the [Transformation Protocol Using Heat Shock]and have plated E.coli cells in LB+Amp plates.
At lunchtime the [bacteria sub-team] and the [yeast sub-team] had a nice meeting to discuss some issues related to the project.
11th July: In this lovely day, the [bacteria sub-team] set up some bacterial cultures, they plated using triple groove in order to get isolated colonies which would be pick and grown in a selective media (37ºC, 200 rpm in a shaking stove) the following day. Moreover, they made new media: LB and LB-agar.
10th July: In this sunny day, the [bacteria sub-team] and the [yeast sub-team] have made an electrophoresis using the DNA which has been digested with EcoRV over-night. The results obtained were uneseful because, as the sub-team members discovered, the enzyme EcoRV was used to introduce the insert into the vector pUC57 so the cleavage site wasn't present anymore (the enzyme EcoRV cuts resulting in blunt ends).
9th July: Today, the [bacteria sub-team] and the [yeast sub-team] have made several mini-preps in order to purify the DNA constructions. The next step has been to test if the mini-preps have been successful. To do it, the sub-teams have digested these constructions (using restriction enzymes: EcoRI, PstI) and they have made an agarose gel in order to do an electrophoresis with the digested DNA. After that, they have revealed the gel and they have found out that the results were unexpected, so they have decided to make another digestion, in this case over-night and using only EcoRI, and carry out the subsequent electrophoresis tomorrow.
Moreover, the [poster sub-team] have made a brain-storming to start designing the logo for the team and the have drawn the first sketches.
8th July: Today, the [bacteria sub-team] and the [yeast sub-team] have transfered our transformed E.coli colonies to liquid media to make them grow over-night to manipulate them tomorrow.
6th July: Today, the [bacteria sub-team] and the [yeast sub-team] have transformed E.coli with the different constructions using the [Transformation Protocol Using Heat Shock]and have plated them in LB+Amp plates.
5th July: Today, the [bacteria sub-team] has finished all the culture media (for each construction). The bacteria sub-team has had lunch with the yeast sub-team and we have discussed some aspects about the project. Also, [yeast sub-team] has made the experimental protocol for next week.
4th July: Today, the [bacteria sub-team] has started to make up their bacteria media (L.B. and defined media).
3th July: Today, the [modelling sub-team] has made a meeting in order to share the deduced equations and decide the definitive ones. Later, it has been decided to search information which could be useful to characterize the behavior of E. coli systems and S. cerevisiae system
21th June: Today, the [modelling sub-team] has made a meeting where the advisors have explained the bases of modelling for synthetic biology. After that, the students have been responsible of deducing the equations that characterize our project.
