Team:NTU-Taida/Human Practice/Information-Platform

From 2012.igem.org

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==Modified P<sub>CI</sub>==
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==Facebook PAGES and Blog==
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<p style="text-indent: 2em;">The circuit incorporates a temperature sensitive cI promoter(CIts) to sense the temperature upshift. In our test we use a thermal adjustable plate reader to detect the mRFP flurorescence. In the beginning, we keep the temperature under 30 Celsius degrees for over 1 hour, and then detect the emission of mRFP. As the dimerized CIts repressor in lower temperature would specifically bind and repress P<sub>CI</sub>, and further hinder the expression of mRFP. We can expect the emission to be low under 610 nm wavelength. We then abruptly increase our temperature to 37 celsius degrees, as we expect the CIts dimer would decompose and lose the function of repressing mRFP expression. </p>
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In order to let more people know about synthetic biology and the competition of iGEM, We not only held several campaigns between other Taiwanese iGEM teams, we also established a Blog (http://igemntuadmin.blogspot.tw/) and a Facebook page (http://www.facebook.com/NationalTaiwanUniversityiGEM) for publics around the world. All Facebook (FB) users have free access to this page. This is one of the attempts We made to promote the visibility of synthetic biology during general public and students of all ages. In order to increase accessibility, we translated the journal article to traditional Chinese. All the articles were posted in traditional Chinese, too. We summited articles about latest advances in synthetic biology and fields relating to iGEM, in a routine schedule, usually two to three articles per week. In sum, we had posted more than 30 articles form July, 2012. The content not only includes latest journal articles sharing, our iGEM NTU_Taida project, the detail process of how we establish our team and the cooperating meetings between us and iGEM team NYMU_Taipei, NCTU_Formosa ,two teams from Taiwan and another team in USA, UIUC-Illinois.  
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[[File:NTU-Taida-Result-Thermal-pCI.png|600px|center]]
[[File:NTU-Taida-Result-Thermal-pCI.png|600px|center]]
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<p style="text-indent: 2em;">The result showed a low level of mRFP expression under wavelength of 580 nm (excitation) and 610 nm (emission). After the sudden temperature upshift, the expression of mRFP steadily rises, and results in 5.7 folds increase in the 8th hour after the temperature upshift. </p>
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<p style="text-indent: 2em;">This proved the fact that repressor CIts and P<sub>CI</sub> can largely lead to increase in protein expression, and can be used in our circuit design as it may turn on the circuit inside human body and spontaneously close down after the bacteria is expelled out. </p>
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Revision as of 13:47, 26 October 2012

NTU-Taida

Information Platform

Human Practice: Information Platform


Contents

Facebook PAGES and Blog

In order to let more people know about synthetic biology and the competition of iGEM, We not only held several campaigns between other Taiwanese iGEM teams, we also established a Blog (http://igemntuadmin.blogspot.tw/) and a Facebook page (http://www.facebook.com/NationalTaiwanUniversityiGEM) for publics around the world. All Facebook (FB) users have free access to this page. This is one of the attempts We made to promote the visibility of synthetic biology during general public and students of all ages. In order to increase accessibility, we translated the journal article to traditional Chinese. All the articles were posted in traditional Chinese, too. We summited articles about latest advances in synthetic biology and fields relating to iGEM, in a routine schedule, usually two to three articles per week. In sum, we had posted more than 30 articles form July, 2012. The content not only includes latest journal articles sharing, our iGEM NTU_Taida project, the detail process of how we establish our team and the cooperating meetings between us and iGEM team NYMU_Taipei, NCTU_Formosa ,two teams from Taiwan and another team in USA, UIUC-Illinois.

NTU-Taida-Result-Thermal-pCI.png



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