Team:Bielefeld-Germany/Amsterdam/Results

From 2012.igem.org

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The iGEM Team successfully produced four active bacterial laccases:
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The iGEM Team successfully produced again four active bacterial laccases:
:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli ''Escherichia coli'' laccase ECOL]
:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli ''Escherichia coli'' laccase ECOL]
:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi''Bacillus pumilus'' laccase BPUL]
:* [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi''Bacillus pumilus'' laccase BPUL]
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All of the bacterial laccases were accomplished to purify. Besides the successfully scale-up fermentation these two laccases could be purified in a high amount to characterize the optimal activity conditions regarding  pH, temperature, buffer solutions  and  organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.
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All of the bacterial laccases were accomplished to purify. The purified laccases . Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol.
The laccase of [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel0 TVEL0 from''Trametes versicolor''] was produced and tested for activity on oaxidation of ABTS.
The laccase of [https://2012.igem.org/Team:Bielefeld-Germany/Results/tvel0 TVEL0 from''Trametes versicolor''] was produced and tested for activity on oaxidation of ABTS.
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Revision as of 15:28, 26 October 2012

Results since Regionals

Summary

coming soon

Laccases

The iGEM Team successfully produced again four active bacterial laccases:

All of the bacterial laccases were accomplished to purify. The purified laccases . Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol. The laccase of TVEL0 fromTrametes versicolor was produced and tested for activity on oaxidation of ABTS.

Immobilization

Coming soon!

For immobilization results see here

Subtrate Analysis

Coming soon!

For more informations click here

Cellulose binding domain

Coming soon!

Read more

Shuttle vector

Coming soon! Read more.


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