Team:Bielefeld-Germany/Amsterdam/Results
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[https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Read more.] | [https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Read more.] | ||
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Revision as of 13:08, 26 October 2012
Summary
coming soon
Laccases
The iGEM Team successfully produced four active bacterial laccases:
All of the bacterial laccases were accomplished to purify. Besides the successfully scale-up fermentation these two laccases could be purified in a high amount to characterize the optimal activity conditions regarding pH, temperature, buffer solutions and organic solvent resistance. Furthermore the iGEM Team Bielefeld demonstrated that the produced laccases can be immobilized maintaining their activity and the degradation capacity was screened for several micro-contaminants. These tests indicate that the ECOL and BPUL are able to degrade ethinyl estradiol and estradiol. The laccase of TVEL0 fromTrametes versicolor was produced and tested for activity on oaxidation of ABTS.
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