Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

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Revision as of 12:39, 20 October 2012

Project Achievements

We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL).
Results

We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest.
Results

We successfully produced purified and identified an active Bacillus pumilus DSM 27 laccase (CotA named BPUL).
Results

We successfully produced, purified an active laccase from Thermus thermophilus HB27 (tthL named TTHL).
Results

We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL).
Results

Successful characterization of the activity of B. pumilus laccase regarding the oxidization of ABTS under different conditions.
Results

Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions.
Results

Successful measurements of enzyme activity of T. thermophilus laccase.
Results

Successful measurements of enzyme activity of B. halodurans laccase.
Results

We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
Results

We successfully immobilized Trametes versicolor-laccase (named TVEL0) and measured their activity.
Results

We successfully immobilized E. coli BL21-laccase (CueO named ECOL) and measured their activity.
Results

We successfully immobilized Bacillus pumilus DSM 27-laccase (CotA named BPUL) and measured their activity. Results

We successfully improved a BioBrick by clarifying its true function.
[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]

We successfully created our own database to organize our samples.
Results

We successfully created a model which simulates the oxidation of ABTS.
Results

We conducted a feasibility study of our project with sewage plant experts.
Results

We did an outstanding outreach work.
Results

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About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

Navigate our Wiki

@@ Line 19: / Line 19: @@
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest. [https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully designed a shuttle vector for site-directed integration in ''P. pastoris'' which allows the production and secretion of a protein of interest.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/vector Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27-Laccase (CotA named BPUL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We successfully produced purified and identified an active ''Bacillus pumilus'' DSM 27 laccase (CotA named BPUL).
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced, purified  an active ''Thermus thermophilus'' HB27-Laccase (tthL named TTHL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced, purified  an active laccase from ''Thermus thermophilus'' HB27 (tthL named TTHL).
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL). [https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   We successfully produced an active ''Bacillus halodurans'' C-125e-Laccase (Lbh1 named BHAL).
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of B. pumilus Laccase regarding the oxidization of ABTS under different conditions. [https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of ''B. pumilus'' laccase regarding the oxidization of ABTS under different conditions.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/pumi#Activity_analysis_of_BPUL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of E. coli Laccase regarding the oxidization of ABTS under different conditions. [https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful characterization of the activity of ''E. coli'' Laccase regarding the oxidization of ABTS under different conditions.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/coli#Activity_Analysis_of_ECOL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful measurements of enzyme activity of T. thermophilus Laccase. [https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful measurements of enzyme activity of ''T. thermophilus'' laccase.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/thermo#Activity_analysis_of_TTHL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful measurements of enzyme activity of B. halodurans Laccase. [https://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]   Successful measurements of enzyme activity of ''B. halodurans'' laccase.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/halo#Activity_Analysis_of_BHAL Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. [https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]]  We established a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analysis showed that our laccases are able to degrade the hormonal substrates estradiol, estrone and ethinyl estradiol.
+[https://2012.igem.org/Team:Bielefeld-Germany/Results/Summary#6 Results]
@@ Line 57: / Line 66: @@
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully improved a BioBrick by clarifying its true function. [http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully improved a BioBrick by clarifying its true function.
+[http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]
-[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created our own database to organize our samples. [https://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]
+[[File:Bielefeld2012_haken_grau.jpg|30px|frameless|left]] We successfully created our own database to organize our samples.
+[https://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]