Team:Bielefeld-Germany/Project/Achievments

From 2012.igem.org

(Difference between revisions)

Revision as of 03:31, 27 September 2012

Project Achievements

We successfully produced purified and identified an active E. coli BL21-Laccase (CueO named ECOL). Results

We successfully designed a shuttle vector for site-directed integration in P. pastoris which allows the production and secretion of a protein of interest. Results

We successfully produced purified and identified an active Bacillus pumilus DSM 27-Laccase (CotA named BPUL). Results

We successfully produced, purified an active Thermus thermophilus HB27-Laccase (tthL named TTHL). Results

We successfully produced an active Bacillus halodurans C-125e-Laccase (Lbh1 named BHAL). Results

We estabilished a method for the degradation of several compounds with the help of our self-produced laccases. The LC-MS and HPLC analytics showed that our laccases are able to degrade the hormonal substrates Estradiol, Esteron and Ethinyl estradiol. Results

We successfully immobilized Trametes versicolor-Laccase (named TVEL0) and measured their activity. Results

We successfully immobilized E. coli BL21-Laccase (CueO named ECOL) and measured their activity. Results

We successfully immobilized Bacillus pumilus DSM 27-Laccase (CotA named BPUL) and measured their activity. Results

We successfully improved a BioBrick by clarifying its true function. [http://partsregistry.org/Part:BBa_K392014:Experience#User_Reviews Results]

We successfully create our own database to organize our samples. Results

55px

About our Wiki

Fifteen students from different fields of study from the Bielefeld University aspired to complete a project called TOXIC COMPOUNDS IN NATURAL WATER - A CASE FOR LACCASE. In his Wiki we document our achievements and our approach.

Contact Us

Universität Bielefeld
Universitätsstraße 25
D-33615 Bielefeld

info(at)igem-bielefeld.de

Navigate our Wiki

Revision as of 03:30, 27 September 2012 (view source) Juvoss (Talk \| contribs) ← Older edit		Revision as of 03:31, 27 September 2012 (view source) Juvoss (Talk \| contribs) Newer edit →
Line 45:		Line 45:


-	[[File:Bielefeld2012_haken_grau.jpg\|30px\|frameless\|left]] We successfully create our own database to organize our samples. [https://2012.igem.org/Team:Bielefeld-Germany/Project/Database]	+	[[File:Bielefeld2012_haken_grau.jpg\|30px\|frameless\|left]] We successfully create our own database to organize our samples. [https://2012.igem.org/Team:Bielefeld-Germany/Project/Database Results]

	</b>		</b>