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Team:HokkaidoU Japan/Notebook/aggregation Week 13
From 2012.igem.org
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| - | ==September 26th== | + | ===September 26th=== |
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| - | ==Characterization of Aggregation== | + | ====Characterization of Aggregation==== |
Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT-pSB1C3 construct. | Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT-pSB1C3 construct. | ||
#Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap. | #Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap. | ||
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#Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager. | #Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager. | ||
| - | Result | + | ''Result''<br /> |
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We couldn't observe eCFP expression, but OD600 of suspension has difference between Arabinose added culture and negative control. | We couldn't observe eCFP expression, but OD600 of suspension has difference between Arabinose added culture and negative control. | ||
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Data(OD600) | Data(OD600) | ||
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|1.221116 | |1.221116 | ||
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{{Team:HokkaidoU_Japan/footer}} | {{Team:HokkaidoU_Japan/footer}} | ||
Revision as of 02:57, 27 September 2012
September 26th
Characterization of Aggregation
Characterization of Aggregation by pBAD-RBS-eCFP-RBS-Ag43-dT-pSB1C3 construct.
- Prepared 5 ml LB liquid medium with appropriate antibiotics into round bottom glass tubes that capped with sponge like cap.
- Suspended colonies into the medium.
- Incubated at 37C for 12 hrs at 180 rpm. For this incubation, pipetted 150 ul of this cultivating medium to 96-well microtiter plate every 2 hrs.
- Analyzed the Absorbance and fluorescence intensity by microtiter plate reader and fluorescence imager.
Result
We couldn't observe eCFP expression, but OD600 of suspension has difference between Arabinose added culture and negative control.
Data(OD600)
| 2(hour) | 4 | 6 | 8 | 10 | 12 |
| Ara+ | |||||
| 0.0119457 | 0.1101659 | 0.013273 | 0.1486576 | 0.2707692 | 0.3663348 |
| Ara- | |||||
| 0.0119457 | 0.1499849 | 0.2813876 | 1.0419305 | 1.1932427 | 1.221116 |
