Team:University College London/HumanPractice/DIYbio/Workshops

From 2012.igem.org

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Prior to the wetlab workshops, we visited the hackspace on several occasions to prepare for the wetlab sessions. This included a short introduction to synthetic biology and our project, health and safety discussion, and primer design workshop. From our early interactions with the citizen scientists, we found that they have done many PCRs and gels before. Some of these have been successful. However, they have not attempted cloning before due to the lack of licensing and UK regulations. What they wanted from this collaboration is to visit our lab to see how we conduct the experiments in an academic institution and also to be able to perform all the steps in the cloning process. Hence, in the UCL workshop schedule we attempted to fit all the experiments in three days.  
Prior to the wetlab workshops, we visited the hackspace on several occasions to prepare for the wetlab sessions. This included a short introduction to synthetic biology and our project, health and safety discussion, and primer design workshop. From our early interactions with the citizen scientists, we found that they have done many PCRs and gels before. Some of these have been successful. However, they have not attempted cloning before due to the lack of licensing and UK regulations. What they wanted from this collaboration is to visit our lab to see how we conduct the experiments in an academic institution and also to be able to perform all the steps in the cloning process. Hence, in the UCL workshop schedule we attempted to fit all the experiments in three days.  
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'''Hackspace Workshop'''
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'''UCL Workshops 3rd-5th September'''
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Hackspace Workshop schedule
Hackspace Workshop schedule
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In the beginning of September, we held three day workshops in UCL teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the biohackers if the pace was slowed down. The amount of practical experience between these citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the biohackers questions and to show the biohackers proper techniques we use in the laboratory.
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UCL Workshops 3rd-5th September
Original UCL Wetlab Workshop schedule  
Original UCL Wetlab Workshop schedule  
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In the beginning of September, we held three day workshops in UCL teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the biohackers if the pace was slowed down. The amount of practical experience between these citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the biohackers questions and to show the biohackers proper techniques we use in the laboratory.
All protocols and results of the collaboration can be found on the London Hackspace wiki ([http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation 1])
All protocols and results of the collaboration can be found on the London Hackspace wiki ([http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation 1])

Revision as of 21:58, 26 September 2012

Contents

DIYbio

Overview | Concept | DIYbio | Workshops | Exhibition | Evaluation | Conclusion

Planning

Prior to the wetlab workshops, we visited the hackspace on several occasions to prepare for the wetlab sessions. This included a short introduction to synthetic biology and our project, health and safety discussion, and primer design workshop. From our early interactions with the citizen scientists, we found that they have done many PCRs and gels before. Some of these have been successful. However, they have not attempted cloning before due to the lack of licensing and UK regulations. What they wanted from this collaboration is to visit our lab to see how we conduct the experiments in an academic institution and also to be able to perform all the steps in the cloning process. Hence, in the UCL workshop schedule we attempted to fit all the experiments in three days.

Hackspace Workshop

Hackspace Workshop schedule

UCL Workshops 3rd-5th September

Original UCL Wetlab Workshop schedule

In the beginning of September, we held three day workshops in UCL teaching laboratory for the citizen scientists. We had an ambitious plan for the workshops, from genomic DNA extraction to ligation and transformation. This was a packed schedule. However, after day one, we quickly realised that it would be a much better learning experience for the biohackers if the pace was slowed down. The amount of practical experience between these citizen scientists also varied. We allowed much longer time intervals between the practicals to answer the biohackers questions and to show the biohackers proper techniques we use in the laboratory.

All protocols and results of the collaboration can be found on the London Hackspace wiki ([http://wiki.london.hackspace.org.uk/view/Public_Biobrick#Visualisation_and_interpretation 1])

Result: Analytical Digest of the Public Biobrick

Analytical digest public biobrick.jpg

'
Five colonies were picked from the ligation plates. After inoculation in LB + chloramphenicol overnight, these were minipreped and analytical digest was performed. All samples in lanes labelled one were digested once with Eco RI and samples labelled two are digested with EcoRI and Pst I. Colonies C and D clearly have the antifreeze insert. Lanes C2 and D2 show bands around 2000bp for the CMP plasmid backbone and around 400bp for the 435bp antifreeze region amplified by the primers. Therefore we have the biobrick!

Safety and regulations

Legality of making competent cell protocol at London Hackspace

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