Team:Goettingen/week17-3

From 2012.igem.org

(Difference between revisions)
Line 29: Line 29:
<b>V08_20_1 Attempt b: increasing the library diversity!</b><br>
<b>V08_20_1 Attempt b: increasing the library diversity!</b><br>
<ul>
<ul>
-
<li>Experiment: <br>A PCR (1000 µL) using the 2<sup>nd</sup> round primers (Tar6973 for and Tar6973 rev) and as template the liquid culture pellet Miniprep that was used at <a href="https://2012.igem.org/Team:Goettingen/week13-3">V07_25</a> as well. protocol according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>. By doing this and later combine the products, the diversity can be increased.</li>
+
<li>Experiment: <br>A PCR (1000 µL) using the 2<sup>nd</sup> round primers (Tar6973 for and Tar6973 rev) and the liquid culture pellet Miniprep that was used at <a href="https://2012.igem.org/Team:Goettingen/week13-3">V07_25</a> as template was performed. Protocol according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>. By doing this and later combine the products, the diversity can be increased. Purification was carried our using the peqGOLD Cycle-pure Kit (PeqLab) with Miniprep Kit columns following the user manual.</li>
</ul>
</ul>
<ul>
<ul>
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<b>V08_20_2 2<sup>nd</sup> round: Plasmid preparation from pellets #1-#4 V08_17 </b><br>
<b>V08_20_2 2<sup>nd</sup> round: Plasmid preparation from pellets #1-#4 V08_17 </b><br>
<ul>
<ul>
-
<li>Experiment: <br>peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H<sub>2</sub>O.</li>
+
<li>Experiment: <br>peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H<sub>2</sub>O. Stored at -20 °C.</li>
</ul>
</ul>
<ul>
<ul>
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#1 - 521 ng/µL<br>
#1 - 521 ng/µL<br>
#2 - 1035 ng/µL<br>
#2 - 1035 ng/µL<br>
-
#3
+
#3 - 2145 ng/µL<br>
 +
#4 - 1103 ng/µL<br>
 +
</li>
 +
</ul>
 +
<br></td></tr>
 +
</table>
 +
<br>
 +
<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
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<tr bordercolor="black" valign="top">
 +
<td width="900" bordercolor="black" valign="top">
 +
<h2><b>V08_20 </b></h2><br>
 +
<b>V08_20_1 Attempt b: increasing the library diversity!</b><br>
 +
<ul>
 +
<li>Experiment: <br>A PCR (1000 µL) using the 2<sup>nd</sup> round primers (Tar6973 for and Tar6973 rev) and the liquid culture pellet Miniprep that was used at <a href="https://2012.igem.org/Team:Goettingen/week13-3">V07_25</a> as template was performed. Protocol according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>. By doing this and later combine the products, the diversity can be increased. Purification was carried our using the peqGOLD Cycle-pure Kit (PeqLab) with Miniprep Kit columns following the user manual.</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>The corresponding agarose gel showed a band of the expected size.</li>
 +
</ul>
 +
<br>
 +
<b>V08_20_2 2<sup>nd</sup> round: Plasmid preparation from pellets #1-#4 V08_17 </b><br>
 +
<ul>
 +
<li>Experiment: <br>peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H<sub>2</sub>O. Stored at -20 °C.</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>DNA concentrations were determined as follows using nanodrop:<br>
 +
#1 - 521 ng/µL<br>
 +
#2 - 1035 ng/µL<br>
 +
#3 - 2145 ng/µL<br>
 +
#4 - 1103 ng/µL<br>
</li>
</li>
</ul>
</ul>
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</table>
</table>
<br>
<br>
-
 
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<br>
<br>

Revision as of 20:04, 26 September 2012

Deutsch  / English 

#3 Chemoreceptor Library - 17th Week

Back to overview

V08_20


V08_20_1 Attempt b: increasing the library diversity!
  • Experiment:
    A PCR (1000 µL) using the 2nd round primers (Tar6973 for and Tar6973 rev) and the liquid culture pellet Miniprep that was used at V07_25 as template was performed. Protocol according to week 10. By doing this and later combine the products, the diversity can be increased. Purification was carried our using the peqGOLD Cycle-pure Kit (PeqLab) with Miniprep Kit columns following the user manual.
  • Observations & Results:
    The corresponding agarose gel showed a band of the expected size.

V08_20_2 2nd round: Plasmid preparation from pellets #1-#4 V08_17
  • Experiment:
    peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H2O. Stored at -20 °C.
  • Observations & Results:
    DNA concentrations were determined as follows using nanodrop:
    #1 - 521 ng/µL
    #2 - 1035 ng/µL
    #3 - 2145 ng/µL
    #4 - 1103 ng/µL


V08_20


V08_20_1 Attempt b: increasing the library diversity!
  • Experiment:
    A PCR (1000 µL) using the 2nd round primers (Tar6973 for and Tar6973 rev) and the liquid culture pellet Miniprep that was used at V07_25 as template was performed. Protocol according to week 10. By doing this and later combine the products, the diversity can be increased. Purification was carried our using the peqGOLD Cycle-pure Kit (PeqLab) with Miniprep Kit columns following the user manual.
  • Observations & Results:
    The corresponding agarose gel showed a band of the expected size.

V08_20_2 2nd round: Plasmid preparation from pellets #1-#4 V08_17
  • Experiment:
    peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H2O. Stored at -20 °C.
  • Observations & Results:
    DNA concentrations were determined as follows using nanodrop:
    #1 - 521 ng/µL
    #2 - 1035 ng/µL
    #3 - 2145 ng/µL
    #4 - 1103 ng/µL


Back to overview
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