Team:Goettingen/week17-3

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<h2><b>V08_20 </b></h2><br>
<h2><b>V08_20 </b></h2><br>
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<b>Attempt b: increasing the library diversity!</b><br>
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<b>V08_20_1 Attempt b: increasing the library diversity!</b><br>
<ul>
<ul>
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<li>Experiment: <br>A PCR using the 2<sup>nd</sup> round primers (Tar6973 for and Tar6973 rev) and as template </li>
+
<li>Experiment: <br>A PCR (1000 µL) using the 2<sup>nd</sup> round primers (Tar6973 for and Tar6973 rev) and as template the liquid culture pellet Miniprep that was used at <a href="https://2012.igem.org/Team:Goettingen/week13-3">V07_25</a> as well. protocol according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>. By doing this and later combine the products, the diversity can be increased.</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>The corresponding agarose gel showed a band of the expected size.</li>
 +
</ul>
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<br>
 +
<b>V08_20_2 2<sup>nd</sup> round: Plasmid preparation from pellets #1-#4 V08_17 </b><br>
 +
<ul>
 +
<li>Experiment: <br>peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H<sub>2</sub>O.</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>DNA concentrations were determined as follows using nanodrop:<br>
 +
#1 - 521 ng/µL<br>
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#2 - 1035 ng/µL<br>
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#3
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</li>
</ul>
</ul>
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Revision as of 18:38, 26 September 2012

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#3 Chemoreceptor Library - 17th Week

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V08_20


V08_20_1 Attempt b: increasing the library diversity!
  • Experiment:
    A PCR (1000 µL) using the 2nd round primers (Tar6973 for and Tar6973 rev) and as template the liquid culture pellet Miniprep that was used at V07_25 as well. protocol according to week 10. By doing this and later combine the products, the diversity can be increased.
  • Observations & Results:
    The corresponding agarose gel showed a band of the expected size.

V08_20_2 2nd round: Plasmid preparation from pellets #1-#4 V08_17
  • Experiment:
    peqGOLD Xchange Plasmid Midi Kit (PeqLab) was used following the user manual. Elution in 100 µL H2O.
  • Observations & Results:
    DNA concentrations were determined as follows using nanodrop:
    #1 - 521 ng/µL
    #2 - 1035 ng/µL
    #3


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