Team:Goettingen/week16-3

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<h2><b>V08_13 </b></h2><br>
<h2><b>V08_13 </b></h2><br>
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<b>Titel</b><br>
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<b>2<sup>nd</sup> round: Mutagenesis PCR, 1000 µL</b><br>
<ul>
<ul>
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<li>Experiment: <br>hier text reinschreiben</li>
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<li>Experiment: <br>The PCR was set up in a volume of 1000 µL following the protocol from <a href="https://2012.igem.org/Team:Goettingen/week10-3">week 10</a>. </li>
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<h2><b>V08_14 </b></h2><br>
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<b>V08_14 2<sup>nd</sup> round: PCR purification</b><br>
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<ul>
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<li>Experiment: <br>The PCR purification was performed using the peqGOLD Cycle-pure Kit (PeqLab), modified using columns from the peqGOLD Plasmid Miniprep Kit (PeqLab)! Elution in 100 µL EB.</li>
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</ul>
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<ul>
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<li>Observations & Results: <br>We now seemed to have found the mistake that caused problems with the loss of DNA material in the second mutagenesis round! Miscommunication led to using the wrong kit for purification steps. We decided to use the peqGOLD Cycle-pure Kit (PeqLab) instead of the subsequent ethanol precipitation as well, as this seemed to rise fewer problems.</li>
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</ul>
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<h2><b>V08_15 </b></h2><br>
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<b>V08_15 2<sup>nd</sup> round: PCR purification</b><br>
 +
<ul>
 +
<li>Experiment: <br>The PCR purification was performed using the peqGOLD Cycle-pure Kit (PeqLab), modified using columns from the peqGOLD Plasmid Miniprep Kit (PeqLab)! Elution in 100 µL EB.</li>
 +
</ul>
 +
<ul>
 +
<li>Observations & Results: <br>We now seemed to have found the mistake that caused problems with the loss of DNA material in the second mutagenesis round! Miscommunication led to using the wrong kit for purification steps. We decided to use the peqGOLD Cycle-pure Kit (PeqLab) instead of the subsequent ethanol precipitation as well, as this seemed to rise fewer problems.</li>
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</ul>
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<br></td></tr>
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</table>
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<br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<br>
<br>

Revision as of 15:28, 26 September 2012

Deutsch  / English 

#3 Chemoreceptor Library - 16th Week

Back to overview

V08_13


2nd round: Mutagenesis PCR, 1000 µL
  • Experiment:
    The PCR was set up in a volume of 1000 µL following the protocol from week 10.


V08_14


V08_14 2nd round: PCR purification
  • Experiment:
    The PCR purification was performed using the peqGOLD Cycle-pure Kit (PeqLab), modified using columns from the peqGOLD Plasmid Miniprep Kit (PeqLab)! Elution in 100 µL EB.
  • Observations & Results:
    We now seemed to have found the mistake that caused problems with the loss of DNA material in the second mutagenesis round! Miscommunication led to using the wrong kit for purification steps. We decided to use the peqGOLD Cycle-pure Kit (PeqLab) instead of the subsequent ethanol precipitation as well, as this seemed to rise fewer problems.

V08_15


V08_15 2nd round: PCR purification
  • Experiment:
    The PCR purification was performed using the peqGOLD Cycle-pure Kit (PeqLab), modified using columns from the peqGOLD Plasmid Miniprep Kit (PeqLab)! Elution in 100 µL EB.
  • Observations & Results:
    We now seemed to have found the mistake that caused problems with the loss of DNA material in the second mutagenesis round! Miscommunication led to using the wrong kit for purification steps. We decided to use the peqGOLD Cycle-pure Kit (PeqLab) instead of the subsequent ethanol precipitation as well, as this seemed to rise fewer problems.


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