Team:TMU-Tokyo/Notebook/Experiment

From 2012.igem.org

(Difference between revisions)

Revision as of 14:29, 26 September 2012

■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)

■Protocols
Plasmid DNA Purification
Genome DNA Purification
Restruction Enzyme Degestion
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium

■Assay
Device1 Assay
Device2 Assay
Device3 Assay

Experiment

Experiment Overview

■Week 0(8.9-8.11)

■9th Aug
Preparation of culture plates with Ampicillin

■10th Aug
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate

■11th Aug
Confirmation of the E.coli growth
Cultivation of the E.coli on LB culture

■12th Aug
Plasmid purification BBa_K299009

■1st week (8.13 - 8.19)
Make Device3→more

■2nd week (8.20 - 8.26)
Make Device1→more

■3rd week (8.27 - 9. 2)
Make Device1 and Make Device3→more

■4th week (9. 3 - 9. 9)
Make all Device→more

■5th week (9.10 - 9.16)
Make all Device→more

■6th week (9.17 - 9.23)
Make all Device→more

■7th week (9.24 - 9.30)
Assay→more and Assay protocol

@@ Line 60: / Line 60: @@
 <Br>
 <div style="margin-left:20px">
-<p class="description3">
+<p class="description">
-<b>■Week 0(8.9-8.11)</b><Br>
+<b>■Week 0(8.9-8.11)</b><Br><p class="description3">
-<Br><b>■ 9th Aug</b><br>
+<Br>
+<b>■9th Aug</b><br>
 Preparation of culture plates with Ampicillin<Br>
-<b>■ 10th Aug</b><br>
+<Br>
+<b>■10th Aug</b><br>
 Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate<Br>
-<b>■ 11th Aug</b><br>
+<Br>
+<b>■11th Aug</b><br>
 Confirmation of the E.coli growth<Br>
-Cultivation of the E.coli on LB culture<Br></p>
+Cultivation of the E.coli on LB culture<Br>
+<Br>
+<b>■12th Aug</b><Br>
+Plasmid purification BBa_K299009<Br>
+<Br>
+</p>
 <Br>
 </div>