Team:Goettingen/week14-3

From 2012.igem.org

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<b>V07_31_2 2<sup>nd</sup> round: <i>Dpn</i>I/<i>Bsa</i>I digestion and purification</b><br>
<b>V07_31_2 2<sup>nd</sup> round: <i>Dpn</i>I/<i>Bsa</i>I digestion and purification</b><br>
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<ul>
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<li>Experiment: <br>The digestion was performed with a total volume of 500 µL according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol</a>. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!</li>
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<li>Experiment: <br>The digestion was performed with a total volume of 500 µL according to <a href="https://2012.igem.org/Team:Goettingen/week10-3">protocol of week 10</a>. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!</li>
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<b>V07_31_3 1<sup>st</sup> round: Ligation</b><br>
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<ul>
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<li>Experiment: <br>The ligation was set up in a 100 µL batch according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Incubation over night at 16 °C.
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</li>
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b>V07_31_2 2

Revision as of 13:33, 26 September 2012

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#3 Chemoreceptor Library - 14th Week

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V07_30


V07_30_1 2nd round: Analysis of the transformation V07_27
  • Experiment:
    The plates and liquid culture were analyzed.
  • Observations & Results:
    Neither the liquid culture nor the plates exhibited bacterial growth.

V07_30_2 2nd round: Saturated mutagenesis PCR, 1000 µL
  • Experiment:
    The PCR was set up again, seeing as we lost our DNA material during the ethanol precipitation the previous week.


V07_31


V07_31_1 2nd round: PCR clean-up
  • Experiment:
    The PCR clean-up was performed according to the established protocol form week 10.
  • Observations & Results:
    The correspodning agarose gel showed a band of the expected size.

V07_31_2 2nd round: DpnI/BsaI digestion and purification
  • Experiment:
    The digestion was performed with a total volume of 500 µL according to protocol of week 10. The purification was now again performed using the peqGOLD Cycle-pure Kit (PeqLab). This should stop the loss of DNA material throughout the purification steps!

V07_31_3 1st round: Ligation
  • Experiment:
    The ligation was set up in a 100 µL batch according to protocol. Incubation over night at 16 °C.


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b>V07_31_2 2

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