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Team:University College London/Module 4/Results
From 2012.igem.org
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== Characterisation of Starvation Promoter BBa_K118011 == | == Characterisation of Starvation Promoter BBa_K118011 == | ||
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Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). | Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). | ||
| + | We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter. | ||
[[File:UniversityCollegeLondon_Buoyancy_K200018.jpg]] | [[File:UniversityCollegeLondon_Buoyancy_K200018.jpg]] | ||
Revision as of 13:22, 26 September 2012
Module 4: Buoyancy
Description | Design | Construction | Characterisation | Modelling | Results | Conclusions
Characterisation of Starvation Promoter BBa_K118011
Normalized expression levels were plotted as relative fluorescent units per optical density at 600 nm. E. coli carrying BBa_K200018 was grown in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 3%, 5%). We carried out further characterization of the pcstA fused with GFP (BBa_K200018) built by Imperial College's 2009 iGem team. Fluorescence was measured from overnight cultures and data collected shows significant higher expression for cultures with 0%, 0.2%, 0.5% glucose concentration, this essay also shows how glucose represses cstA promoter.
