Team:Goettingen/week7-1

V06_11

• Experiment:
  A variety of agar conditions were tested: 1) 1% trypton, 0.3% agar, 0.5% NaCl; 2) 0.5% trypton, 0.3%, 0.5% NaCl; 3) like 1, but with additionally 0.5% glcose; 4) like 2), but with additionally 0.5% glucose. The swimming assay was performed as mentioned in methods.
• Observations & results:
  All plates w/o glucose showed strong swimming over night. Therefore, glucose could be considered to be taxis-inhbiting.

V06_12

• Experiment:
  The strains from the previos experiments were combined on a single plate (several distinct drops on the same plate) to compare their swimming ability directly.

V06_13

• Experiment:
  Two strains with different promotor strengths were mixed 1:1 (OD600) and dropped onto a trypton plate. After several hours of swimming, the bacteria from the most outer edge of the swimming halo were picked and quantified to identify the ratio between the both starter-strains.
• Observations & Results:
  The plated did not show enough swimming, to ensure a positiv separation of the two strains. Further incubation times might be helpful.

• Experiment:
  Two strains with different promotor strengths were mixed 1:1 (OD600) and dropped onto a trypton plate. After several hours of swimming, the bacteria from the most outer edge of the swimming halo were picked and quantified to identify the ratio between the both starter-strains.


• Observations & Results:
  On each quater of a 150 mm petri dish was one bacteria colony droped. Therefore, the swimming ability of all four strains could be compared directly.

• Experiment:
  On minimal plates (only 0.3% agar and 0.5% NaCl) was one strain dropped. In a distance of about 2 cm was an attractant dropped, to induce directed chemotaxis.


• Observations & Results:
  No directed swimming or swimming at all was detectable. The aspartat attractant might not have reached the bacteria.