Team:TMU-Tokyo/Notebook/Experiment
From 2012.igem.org
Line 59: | Line 59: | ||
<p class="description"> | <p class="description"> | ||
+ | <b>■Week 0(8.9-8.11)</b><Br> | ||
+ | 9th Aug<Br> | ||
+ | Preparation of culture plates with Ampicillin<Br> | ||
+ | 10th Augt<Br> | ||
+ | Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate<Br> | ||
+ | 11th Augt<Br> | ||
+ | Confirmation of the E.coli growth<Br> | ||
+ | Cultivation of the E.coli on LB culture<Br> | ||
+ | |||
<b>■1st week (8.13 - 8.19)</b><Br> | <b>■1st week (8.13 - 8.19)</b><Br> | ||
Make Device3→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Experiment/1st week (8.13 - 8.19)">more</A></p> | Make Device3→<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook/Experiment/1st week (8.13 - 8.19)">more</A></p> |
Revision as of 12:43, 26 September 2012
■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)
■Protocols
Plasmid DNA Purification
Genome DNA Purification
Restruction Enzyme Degestion
DNA Fragment Ligation
Transformation
Electrophoresis
LB Medium
■Assay
Device1 Assay
Device2 Assay
Device3 Assay
Experiment
■Week 0(8.9-8.11)
9th Aug
Preparation of culture plates with Ampicillin
10th Augt
Cultivation of transformed E.coli (BBa_K299009: ordered previously) on plate
11th Augt
Confirmation of the E.coli growth
Cultivation of the E.coli on LB culture
■1st week (8.13 - 8.19)
Make Device3→more
■2nd week (8.20 - 8.26)
Make Device1→more
■3rd week (8.27 - 9. 2)
Make Device1 and Make Device3→more
■4th week (9. 3 - 9. 9)
Make all Device→more
■5th week (9.10 - 9.16)
Make all Device→more
■6th week (9.17 - 9.23)
Make all Device→more