Team:Goettingen/week13-3

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Revision as of 15:10, 24 September 2012


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2^nd round: Analysis of plates and liquid culture V07_20

Experiment:
Both the liquid culture and the plates exhibited bacterial growth. 14 clones were transferred to a 5 mL liquid culture (LB + CM) and incubated over night at 37 °C, 180 rpm. The liquid culture was centrifuged at 4800 rpm, 4 °C, 10 min. The pellet was stored at -20 °C.

V07_25_1 2^nd round: Plasmid preparation clones 1-14 for subsequent sequencing

Experiment:
Plasmid preparation was performd with peqGOLD Plasmid Miniprep Kit (PeqLab) following the user manual. DNA concentrations were determined via nanodrop for subsequent sequencing.

V07_25_2 2^nd round: Saturated mutagenesis PCR 200 µL (repeat)

Observations and results:
The corresponding agarose gel showed bands of the expected sizes.

2^nd round:

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 <li>Experiment: <br>The PCR was set up following the protocol from week <a href="https://2012.igem.org/Team:Goettingen/week10-3">10</a>. </li>
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 <ul>
 <li>Observations and results: <br>The corresponding agarose gel showed bands of the expected sizes. </li>